3.2.4.2 Clinical Enzymology (Alkaline Phosphatase, ALP Determination)

Question 1

Specimen Consideration for Alkaline phosphatase:

• Avoid hemolysis
• Avoid Chelating agents
• Avoid prolonged storage

Which among the following does not belong

Answer 1

None of the above

• Priority is chelating agents since Mg is important in reaction
• Prolong storage may lead to pH changes

Question 2

Reference method for Alkaline phosphatase?

a. Hexokinase
b. Bowser-McComb
c. Bowers-McComb
d. McHarly-Bowers

Answer 2

c

Question 3

What is being determined in Bowers-McComb:

a. p-nitrophenylphosphate
b. p-nitrophenol
c. Phosphate
d. H2O

Answer 3

b

Question 4

What is the color and absorbance for p-nitrophenol?

a. Yellow, 340nm
b. Yellow, 405nm
c. Blue, 340nm
d. Blue, 405nm

Answer 4

b

Question 5

What is the substrate in Bowers-McComb:

a. p-nitrophenylphosphate
b. p-nitrophenol
c. Phosphate
d. H2O

Answer 5

a

Question 6

All other methods in ALP determination has the same substrate, color produce, and absorbance

T or F

Answer 6

T

Question 7

Reference value for ALP

a. 30-120 U/L
b. 30-60 U/L
c. 30-90 U/L
d. 30-80 U/L

Answer 7

c