2.4.6 Proteins (Lab determination of Proteins)

Question 1

Measures Refractive index due to proteins in solution

a. Kjeldahl Method
b. Refractometry Method
c. Turbidimetry
d. UV absorption

Answer 1

b

Question 2

Ultimate reference method based on measuring nitrogen content

a. Kjeldahl Method
b. Refractometry Method
c. Turbidimetry
d. UV absorption

Answer 2

a

Question 3

Change in optical density followin precipitation with TRICHLOROACETIC ACID (TCA, Sulfosalicylic acid (SSA), Benzalkonium chlorinde

a. Kjeldahl Method
b. Refractometry Method
c. Turbidimetry
d. UV absorption

Answer 3

c

Question 4

UV absorption:

Measurement of absorbance at _____ owing mostly to Tryptophan, Tyrosine, and Phenylalanine

Answer 4

280 nm

Question 5

Biuret method:

Detection of peptide bonds, forming a _______ which is measured at _____ nm

a. Violet, 540nm
b. Blue, 500nm
c. Green, 400nm
d. Red, 700nm

Answer 5

a

Question 6

Biuret method which is a colorimetric method has a reagent that comprises of:

• Copper sulfate
• Rochelle salt
• NaOH
• Potassium iodide

Which of the following does not belong?

Answer 6

None

Question 7

Oxidation of phenolic groups

a. Folin-Ciocalteau (Lowry)
b. Ninhydrin Method (Colorimetric)
c. Dye-binding technique

Answer 7

a

Question 8

Lowry method oxidized phenolic groups producing what color?

a. deep Violet
b. deep Blue
c. deep Red
d. deep Green

Answer 8

b

Question 9

Lowry is more sensitive than biuret

T or F

Answer 9

T

Question 10

Formation of VIOLET color in the presence of AMINO acid

a. Folin-Ciocalteau (Lowry)
b. Ninhydrin Method (Colorimetric)
c. Dye-binding technique

Answer 10

b

Question 11

Dye-binding technique

What is the dye used for total protein?

a. Coomassie Brilliant blue
b. Ponceus S
c. Amido black
d. Methyl Orange

Answer 11

a

Question 12

Dye used for Albumin

a. Coomassie Brilliant blue
b. Ponceus S
c. Amido black
d. Methyl Orange

Answer 12

d

(Nonspecific)

Question 13

The following other dyes is used in the detection of albumin:

• 2,4 HABA
• BCG
• BCP

Which among the following is most common

Answer 13

BCG

Question 14

The following other dyes is used in the detection of albumin:

• 2,4 HABA
• BCG
• BCP

Which among the following is specific, sensitive and precise?

Answer 14

BCP

Question 15

The following other dyes is used in the detection of albumin:

• 2,4 HABA
• BCG
• BCP

Which among the following is subject to interference

Answer 15

2,4 HABA

Question 16

Reference Values:

6.5 - 8.3 g/dL

a. TP, Ambulatory
b. Albumin

Answer 16

a

Question 17

Reference Values:

3.5 - 5.5 g/dL

a. TP, Ambulatory
b. Albumin

Answer 17

b

Question 18

A/G Ratio = A/(TP-A)

What is the normal value?

a. 1.1 - 2.0
b. 1.0 - 2.1
c. 1.1 - 2.1

Answer 18

a

Question 19

If low/inverted ratio (low Alb, high immunoglobulin), it indicates monoclonal gammopathy (multiple myeloma & Waldenstrom’s macroglobulinemia)

T or F

Answer 19

T