3.1.2 Enzymology Intro (Enzyme kinetics) Flashcards

1
Q

E + S <-> ES <-> P + E

Is this the correct enzymatic reaction?

T or F

A

T

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2
Q

[E] > [S]

a. First-order kinetics
b. zero-order kinetics

A

a

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3
Q

[S] > [E]

a. First-order kinetics
b. zero-order kinetics

A

b

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4
Q

[E] the bracket indicates?

a. Time
b. Concentration

A

b

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5
Q

IF:

[E] > [S]

What is the rate-limiting component?

A

[S]

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6
Q

IF:

[E] > [S]

What should you add to hasten the reaction?

a. Enzyme
b. Substrate

A

b

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7
Q

If there are no available active sites on the enzymes, they will be saturated and cannot continue to reaction

a. First order
b. Zero-order

A

b

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8
Q

Enzyme activity is being measured in Enzyme kinetics and not the concentration

T or F

A

T

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9
Q

V = rate of reaction
Km = [S] at 1/2 Vmax

V = (Vmax . [S]) / (Km + [S])

a. Michaelis-Menten equation
b. Lineweaver-Burke plot

A

a

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10
Q

Point where there are no available active site of enzymes

a. Max velocity
b. 1/2 max velocity

A

a

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11
Q

Linear phase

a. 1st order
b. zero order

A

a

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12
Q

Plateau phase

a. 1st order
b. zero order

A

b

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13
Q

Km = Unique for each Enzymes

T or F

A

T

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14
Q

Double reciprocal plot of the Michaelis-Menten equation

a. Michaelis-Menten equation
b. Lineweaver-Burke plot

A

b

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15
Q

1/v = (Km/Vmax) x (1/[S]) + (1/Vmax)

a. Michaelis-Menten equation
b. Lineweaver-Burke plot

A

b

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16
Q

y = mx + b

1/v

a. y
b. m
c. x
d. b

A

a

17
Q

y = mx + b

Km/Vmax

a. y
b. m
c. x
d. b

A

b

18
Q

y = mx + b

1/[s]

a. y
b. m
c. x
d. b

A

c

19
Q

y = mx + b

1/Vmax

a. y
b. m
c. c
d. b

A

d