3.1.2 Enzymology Intro (Enzyme kinetics) Flashcards
E + S <-> ES <-> P + E
Is this the correct enzymatic reaction?
T or F
T
[E] > [S]
a. First-order kinetics
b. zero-order kinetics
a
[S] > [E]
a. First-order kinetics
b. zero-order kinetics
b
[E] the bracket indicates?
a. Time
b. Concentration
b
IF:
[E] > [S]
What is the rate-limiting component?
[S]
IF:
[E] > [S]
What should you add to hasten the reaction?
a. Enzyme
b. Substrate
b
If there are no available active sites on the enzymes, they will be saturated and cannot continue to reaction
a. First order
b. Zero-order
b
Enzyme activity is being measured in Enzyme kinetics and not the concentration
T or F
T
V = rate of reaction
Km = [S] at 1/2 Vmax
V = (Vmax . [S]) / (Km + [S])
a. Michaelis-Menten equation
b. Lineweaver-Burke plot
a
Point where there are no available active site of enzymes
a. Max velocity
b. 1/2 max velocity
a
Linear phase
a. 1st order
b. zero order
a
Plateau phase
a. 1st order
b. zero order
b
Km = Unique for each Enzymes
T or F
T
Double reciprocal plot of the Michaelis-Menten equation
a. Michaelis-Menten equation
b. Lineweaver-Burke plot
b
1/v = (Km/Vmax) x (1/[S]) + (1/Vmax)
a. Michaelis-Menten equation
b. Lineweaver-Burke plot
b