3.2.2.2 Clinical Enzymology (Lactate Dehydrogenase, LD determination) Flashcards

1
Q

LDH specimen consideration:

  • Avoid hemolysis
  • Avoid cold storage
  • Avoid chelating agent

Which of the following does not belong?

A

None

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2
Q

LDH is the most affected enzyme by hemolysis

T or F

A

T

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3
Q

Which among the isoenzymes of LDH is the most cold-labile

a. LD1
b. LD2
c. LD3
d. LD5

A

d

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4
Q

Forward

a. Wacker
b. Wroblewski-Ladue

A

a

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5
Q

reverse reaction

a. Wacker
b. Wroblewski-Ladue

A

b

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6
Q

Lactate + NAD -(LDH)-> Pyruvate + NADH + H

a. Wacker
b. Wroblewski-Ladue

A

a

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7
Q

Pyruvate + NADH + H -(LDH)-> Lactate + NAD

a. Wacker
b. Wroblewski-Ladue

A

b

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8
Q

Reference value for LDH

a. 200-250 U/L
b. 200-225 U/L
c. 100-200 U/L
d. 100-225 U/L

A

d

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9
Q

MI Protein Markers:

a. Myoglobin
b. Troponin I
c. Fetoprotein
d. Cardioprotein

A

a and b

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10
Q

MI Markers:

Sequence of elevation

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

a,b,c,d,e

(MICAL)

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11
Q

MI Markers:

Elevation: 1-3hrs
Peaks: 5-12 hrs

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

a

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12
Q

MI Markers:

Elevation: 12-24hrs
Peaks: 48-72 hrs

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

e

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13
Q

MI Markers:

Elevation: 3-4hrs
Peaks: 14-20 hrs

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

b

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14
Q

MI Markers:

Elevation: 6-8hrs
Peaks: 18-24 hrs

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

d

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15
Q

MI Markers:

Elevation: 4-6hrs
Peaks: 12-24 hrs

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

c

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16
Q

MI Markers:

Normal: 18 -30hrs

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

a

17
Q

MI Markers:

Normal: 5-20 days

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

b

18
Q

MI Markers:

Normal: 2-3 days

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

c

19
Q

MI Markers:

Normal: 4-5 days

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

d

20
Q

MI Markers:

Normal: 10 days

a. Myo
b. Tn (i)
c. CK
d. AST
e. LD

A

e

21
Q

___________ is the gold standard since it can sustained elevation or a week

a. Myo
b. Tropnin I
c. CK
d. LD

A

b