3.2.3.2 Clinical Enzymology (Aminotransferases/Transaminases, AST/ALT Determination) Flashcards

1
Q

Specimen consideration for AST/ALT includes:

  • Avoid prolonged storage
  • Avoid Hemolysis
  • Avoid chelating agent

Which of the following does not belong

A

Avoid chelating agent

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2
Q

In Reitman-Frankel, it is a colorimetric-spectrophotometric assay

T or F

A

T

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3
Q

Uses 2,4 dinitrophenulhydrazine and 0.4 NaOH

a. Reitman-Frankel
b. Coupled Enzymatic Reaction

A

a

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4
Q

In Reitman-Frankel If AST is used as catalyst, what is the substrate?

a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate

A

a

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5
Q

In Reitman-Frankel If AST is used as catalyst, what is the Reagent?

a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate

A

c

both AST and ALT

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6
Q

In Reitman-Frankel If ALT is used as catalyst, what is the substrate?

a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate

A

b

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7
Q

In Reitman-Frankel If AST is used as catalyst, what is the Product?

a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate

A

e

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8
Q

In Reitman-Frankel If ALT is used as catalyst, what is the Product?

a. Aspartate
b. Alanine
c. Alpha ketoglutarate
e. Oxaloacetate
f. Pyruvate

A

f

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9
Q

Which among is the color developer for Reitman-frankel?

a. 2,4 DNPH
b. 2,4 Dinitrophenylhydrazone

A

a

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10
Q

Based on Karmen method

a. Reitman-Frankel
b. Coupled Enzymatic Reaction

A

b

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11
Q

What is the coupling enzyme for AST?

a. Malate dehydrogenase
b. Lactate dehydrogenase

A

a

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12
Q

What is the coupling enzyme for ALT?

a. Malate dehydrogenase
b. Lactate dehydrogenase

A

b

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13
Q

What is being produce for coupled Enzymatic reaction?

a. NAD
b. NADH
c. NADP
d. NADPH

A

a

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14
Q

Reference value:

5-35 U/L

a. AST
b. ALT

A

a

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15
Q

Reference value:

7-45 U/L

a. AST
b. ALT

A

b

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