4.4.2 Toxicology (Instrumentation) Flashcards
A semi quantitative screening test that is used for Interpreation of results utilized Rf values of solutes in comparison to aqueous standard
a. Thin layer chromatography
b. Liquid chromatography
c. Gas Chromatography
d. Mass spectrometry
a
Rg is the distance migrated by a sample componets/distance migrated
by the solvent
T or F
T
For insufficiently volatile and thermolabile compounds
a. Thin layer chromatography
b. Liquid chromatography
c. Gas Chromatography
d. Mass spectrometry
b
It is the polar stationary phase and non polar mobile phase
a. HPLC
b. Normal phase LC
c. Reverse phase LC
b
It is a nonpolar stationary phase and polar mobile phase
a. HPLC
b. Normal phase LC
c. Reverse phase LC
c
is a type of liquid chromatography where the mobile phase is a liquid that is passed over the stationary phase of the column
a. HPLC
b. Normal phase LC
c. Reverse phase LC
a
Common detector system in GC or HPLC
a. Thin layer chromatography
b. Liquid chromatography
c. Gas Chromatography
d. Mass spectrometry
d
Useful for compounds that are naturally volatile or can easily be converted into a volatile form
a. Thin layer chromatography
b. Liquid chromatography
c. Gas Chromatography
d. Mass spectrometry
c
It takes for a compound to elute in GC or HPLC.
a. Retention time (tr)
b. Peak area/height
a
it is used as basis for identification of a solute
a. Retention time (tr)
b. Peak area/height
a
It is the proportional to the amount of solute and it is used for Quantification
a. Retention time (tr)
b. Peak area/height
b
It produces a signal for identification and quantification of the solutes
a. Thin layer chromatography
b. Liquid chromatography
c. Gas Chromatography
d. Mass spectrometry
d
A homogenous assay in which the activity of an enzyme as a label is inhibited by the binding of antibody to antigen conjugated with enzyme
a. Microparticle enzyme immunoassay (MEIA)
b. Enzyme multiplied immunoassay technique (EMIT)
c. Fluorescence polarization immunoassay (FPIA)
b
it uses microparticles as solid phase, an enzyme label and a fluorogenic substrate
a. Microparticle enzyme immunoassay (MEIA)
b. Enzyme multiplied immunoassay technique (EMIT)
c. Fluorescence polarization immunoassay (FPIA)
a
uses polarized light to excite a fluorescent label and it is the rate of rotation is inversely proportional to the degree of polarization and analyte concentration
a. Microparticle enzyme immunoassay (MEIA)
b. Enzyme multiplied immunoassay technique (EMIT)
c. Fluorescence polarization immunoassay (FPIA)
c
