BL - Immunodiagnosis Flashcards Preview

BL/DD USMLE and Basics > BL - Immunodiagnosis > Flashcards

Flashcards in BL - Immunodiagnosis Deck (12):

To test the humoral arm of the immune system, we begin with:

serum protein electrophoresis


B cells in blood can be measured by counting cells with:

surface immunoglobulin
CD19 or CD20


The best overall test (for Th1 activity): skin test with common antigens to which most people
will have

DTH- Delayed-Type Hypersensitivity


Antibodies against autoantigens in the nucleus are best observed using human cells grown on a slide.

Antinuclear antibodies


(IgM anti-IgG) is detected by its ability to agglutinate latex particles if they
have been coated with IgG

Rheumatoid factor


Sample of serum in the fridge examined after 1 – 7 days for a precipitate;

this precipitate is called a mixed cryoglobulin, to distinguish it from the pure (monoclonal) cryoglobulin seen in multiple myeloma.

Immune complexes-Type III disease


can be used to identify antibody in a patient’s tissues (direct immunofluorescence) or in their blood (indirect).



like IF, but uses a final antibody labeled instead with an enzyme, typically peroxidase, which produces a brown or black product.

Slides can be observed with an ordinary and archived



done in the HIV antibody screen, antigen is coupled to a plate, then the test serum is added; if there is antibody to the antigen it will bind. It is then identified using an enzyme-coupled antibody to the specific class of the expected serum antibody

Simple ELISA


If an antigen is at least divalent, the favorite technique is a sandwich or______

capture ELISA


obtain sets of tiny latex beads coated
separately with antibodies to 4 common bacterial culprits1. Add beads to a drop of the patient’s cerebrospinal fluid; if any agglutinate, it’s because they have been cross-linked by bacterial antigen that was in the CSF.

Reverse passive agglutination.


Small molecules, with only one epitope, can’t be measured in a ______

capture assay.