DD- Virology of RSV

Question 1

RSV

Answer 1

Human respiratory syncytial virus (RSV)

Question 2

RSV

Answer 2

a virus that causes respiratory tract infections. It is a major cause of lower respiratory tract infections and hospital visits during infancy and childhood.

Question 3

RSV Epidemiology

Answer 3

Large particle droplet spread
Very high (40-60%) attack rate children < 2 years
Most serious illness in very young, specific high-risk populations

Question 4

is there an rsv vaccine?

Answer 4

no

Question 5

RSV structure- ________ family

Answer 5

paramyxoviridae

Question 6

RSV structure genome

Answer 6

Single-stranded, nonsegmented RNA virus

Question 7

RSV
Fusion of viral envelope to host cell
Fusion of membranes of infected cells to each other “syncytia”

Answer 7

F protein

Question 8

RSV

Initial binding of virus to host cell

Answer 8

G protein:

Question 9

RSV subtypes

Answer 9

A and B

A causes most disease

Question 10

RSV Pathophysiology- Invades:

Answer 10

conjunctiva/nasopharynx

Question 11

RSV causes severe damage to the epithelium and _________apparatus

Answer 11

bronchiolar ciliary

*Results in collection of fluid in the bronchioles and alveoli

Question 12

RSV Pathophysiology _________incubation period

Answer 12

3-5 day

Question 13

Clinical Picture or RSV

Answer 13

Respiratory distress, wheeze/rhonchi, hypoxia, copious secretions: Bronchiolitis

Question 14

RSV causes severe damage to the _________and bronchiolar ciliary apparatus

Answer 14

epithelium

*Results in collection of fluid in the bronchioles and alveoli

Question 15

Testing for RSV

Answer 15

Cell culture – takes more time

Direct antigen detection (rapid test) – not very sensitive/accurate

Nucleic acid/Genomic amplification (PCR) – most accurate and quick in many
places

Question 16

do people get recurrent infections of RSV?

Answer 16

Yes

Question 17

RSV _______ like influenza to avoid immune system

Answer 17

“drifts”

Question 18

RSV has been associated with _________later in life, but whether it causes it is unclear

Answer 18

wheezing & asthma

Question 19

Is there an RSV vaccine?

Answer 19

no

vaccine attempts made disease worse

Question 20

RSV vaccine recipients were not protected against disease and they had worse RSV disease when they got infected with RSV.

Hypothesized that serum antibody without local respiratory antibody may _________disease.

Answer 20

worsen

Question 21

RSV immunoprophylaxis with Palivizumab (Respigam)

Answer 21

Respigam is a human pooled antibody with high RSV titers, shown to have decrease in disease severity and hospitalization

Question 22

A mouse monoclonal anti-RSV antibody was developed

Answer 22

Palavisumab

Synagis

Question 23

RSV vaccine recipients were not protected against disease and they had _____RSV disease when they got infected with RSV.

Hypothesized that serum antibody without local respiratory antibody may _________disease.

Answer 23

worse

worsen

Question 24

Antibody/immune protection is _________for infection, but previous infection may prevent more severe disease

Answer 24

incomplete