Flashcards in ID 3 Deck (55)
Function of type III secretion system?
facilitates direct delivery of toxins from certain gram negatives to eukaryotic host cells.
bugs with type III secretion systems?
4) e coli
other name for transformation?
bugs with transformation ability
1) s pneumo
2) H flu
How can you prevent transformation?
Has genes required for sex plus and conjugation
What is transferred through piles in conjugation?
Single strand of plasmid DNA. NO chromosomal DNA.
Cell that has incorporated F+ plasmid into bacterial chromosomal DNA.
Caveat about Hfr Cells
When plasmid DNA is replicated, some flanking chromosomal DNA can be replaced. Thus, Hfr cells can transfer BOTH plasmid and chromosomal genes.
Between plasmid and chromosome and vice versa
flanking chromosomal DNA can be transferred to another bacterium
example of transposition
vanA gene transferred from VRE to S aureus
A "packaging" event. Lytic phage infects bacterium, leading to cleavage of bacterial DNA. Parts of bacterial chromosomal DNA may become packaged in phage capsid. Phage then infects another bacterium --> genes transferred.
"Excision event." Lysogenic phage infects bacterium; viral DNA incorporates into bacterial chromosome --> phage DNA excised --> flanking bacterial genes may be excised with it --> DNA is packaged into phage capsid and can infect another bacterium.
Genes from what toxins are encoded in a lysogenic phage?
Group A strep erythrogenic toxin
when do bacteria form spores?
End of stationary phase
how do you kill spores?
autoclave by steaming at 121 C for 15 minutes.
where are exotoxin genes?
plasmid or bacteriophage
potency of exotoxins vs. endotoxins
Exotoxins are VERY dangerous (fatal dose on order of 1 microgram)
Endotoxins are less potent (fatal dose on order of hundreds of micrograms)
antigenicity of exotoxins?
Induces high-titer antibodies called antitoxins.
what is titer?
way of expressing concentration
vaccines against exotoxins?
heat stability of exotoxins?
Destroyed rapidly at 60 degrees C (except staphylococcal enterotoxin)
typical exotoxin diseases
source of endotoxins?
outer cell membrane of gram negatives
composition of endotoxins?
Lipid A component of LPS
Effects of endotoxins?
cytokines induced by endotoxin?