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Flashcards in Microscopy ID Deck (25):
1

What are the steps in a Gram stain preparation?

1. Mount the specimen on the slide

2. Stain with crystal violet

3. Use iodine as a mordant so the color isn't washed out

4. EtOH/Acetone wash (Gram + will stay violet)

5. Counterstain with safari (Gram - will stain pink)

2

What are the best specimens for a direct exam?

-Sputum

-Urine

-CSF

-Vaginal Smear

3

What are Acid Fast stains used for?

They are used for bacteria with cell walls of long chains of fatty acids

4

What are examples of bacteria that Acid Fast stains?

-Mycobacteria

-Nocardia

-Cyclospora

-Cryptosporidia

5

What are the 4 fungal stains?

Lactophenol Cotton Blue

GMS

PAS

Mucicarmine

6

Lactophenol Cotton Blue stains....

Histoplasma

7

GMS stains...
 

Yeast cells and hyphae

Pneumocystis carinii

Aspergillus

8

PAS stains...

Yeast cells and hyphae

Aspergillus

9

Mucicarmine stains...

Cryptococcus

10

What are the parasite stains?

Wright Giemsa

Wet Mounts

Trichrome

Modified Acid Fast

11

What is the Warthin-Starry stain?

Silver stain for bacteria that stains spirochetes and cat scratch disease

12

Wright Giemsa stains...

Histoplasma

Leishmania

Trypanosomas

Malaria

13

Wet Mounts stain...

Giardia

14

Trichrome stains...

Giardia

15

What are the fluorescence stains?

Auramine-Rhodamine

Calcofluor White

Flourescein Conjugated Antibodies

16

Auramine-Rhodamine stains...

Mycobacteria

17

Calcofluor White stains...

 

Fungi

18

Flourescein Conjugated Antibodies stain...

Viruses

Pneumocystis

Bordetella

Legionella

19

Which one of the following stains is most useful for demonstrating Aspergillus in lung tissue?

Modified Acid-Fast

Trichrome

Wright’s Giemsa

Gomori Methenamine Silver

Gram Stain 

Gomori Methenamine Silver

20

Staphylococcus aureus 

21

Neisseria meningitides in CSF

22

E. coli in blood

23

Actinomyces

24

Campylobacter in jejunum

25

Nocardia

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