Objectives
Why should a physician care about cell movement
The Cytoskeleton
Cytoskeletal Proteins
Cytoskeletal Functions
What is the green?
What are Microtubules
Microtubule Structure
Video of Microtubules
Lysosomes and Endosomes Revisited
Kinesins vs. Dyneins
Centrioles
Mitotic Spindle
Name 3 Drugs that Affect Microtubule function
- Colchicine
- Vinblastine
- Taxol
Colchicine
Vineblastine
Taxol
Cilia Structure and Function
Primary Cilliary Dyskinesia
Microfilament Structure
Polymorphism of Microtubules
Microfilaments and Cytoskeleton
Microvilli vs. Cillia
Microvilli — shorter than cilia and immobile. Their core protein of Microfilaments
Cilia — core protein of microtubules — actively moved by the cell
Cell Division and Microtubules vs. Microfilaments
Name 2 Drugs that Affect Microfilament Function
Cytochalasins
Phalloidin
Intermediate Filaments Structure and Function
Type I intermediate Filaments
Type II Intermediate Fillaments
Type III Intermediate Filaments
Type IV Intermediate Filaments
Type V Intermediate Filaments
Intermediate Filaments and Structural Integrity
Skin Cells Stained for Keratin
Epidermolytic Bullosa Simplex (EBS)
EBS Mutation
Epidermolytic Hyperkeratosis
Epidermolytic Palmoplantar Keratoderma
Hypertrophic Cardiomyopathy
Motor Neuron Disease
NF-H Overexpression
Taxol is used to treat which of the following?
Broken Bones
Cancer
Atrophy of Muscle
Viral Infections
Bacterial Infections
Cancer
Which of these are involved in chromosome movement during mitosis and meiosis?
Microtubules
Intermediate filaments
Microfilaments
Microtubules
Which of these are involved in cytokinesis?
Microtubules
Intermediate filaments
Microfilaments
Microfilaments
What is the diameter and polarity of microtubules?
50nm Plar
50 nm Non polar
25nm Polar
25nm Polar
Which is true regarding cytoskeletal Elements and subunits?
Most cytoskeletal proteins are part of the network
Most cytoskeletal proteins are soluble subunits
There is a balance between network and soluble subunits in the cell.
There is a balance between network and soluble subunits in the cell.
Cytoskeletal elementas have a wide variety of functions much of this variety of function can be explained by?
Different core subunits in each element
Polarity of each element
The protein associated with each element
Availability of ATP to each Element
The protein associated with each element
Genetic defects in keratin genes leads to which of the following?
Cardiomyopathy
Mental Retardation
Hepatitis
Early onset diabetes
Skin blistering of newborns
Skin blistering of newborns
If you had a sample of tumor from a patient and whereintereste in knowing the origin of the tumor cells an antibody to which protein family would be most helpful?
Microtubules
Intermediate Filaments
Microfilaments
Intermediate Filaments
Which of these are the “Tracks” on which cellular vesicles travel?
Microtubules
Intermediate Filaments
Microfilaments
Microtubules
What is the diameter and polarity of intermediate filaments?
10 nm Polar
10nm Non Polar
10nm Non Polar
Which of the following describing the microtubule organizing center is correct?
Near golgi associated with the minus end of microtubules
Near golgi associated with the plus end
Near nucleus associated with the minus end of the microtubules
Near the nucleus associated with the plus end of the microtubules
Near golgi associated with the minus end of microtubules
In this image green is staining for tubular the red dot is which of the following?
Centriole
Filopdia
Mitochondria
The endoplasmic reticulum
Centriole
What is the diameter and polarity of microfilaments?
5nm Polar
Which of the following forms the core protein in cillia
Microtubules
Intermediate Filaments
Microfilaments
Microtubules