CILS Molecular Techniques Pt. II Flashcards Preview

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Flashcards in CILS Molecular Techniques Pt. II Deck (17):
1

 

True or False?

If a researcher wants to determine if a genes is expressed in cells in a tissue section, the quesiont can be fully answered using either immunohistochemisitry or in in situ hybridization?

 

 

 

False — You need to know whether not the protein is present because the mRNA could be present but proteins not made

2

 

 

True or False?

Infections of virus and bacteria increase risk for cancer?

 

 

True

3

 

 

 

The RB protein acts as a brake stopping uncontrolled progression?

 

 

 

 

True

4

 

 

 

RB protein is both a co-repressor and a tumor supressor?

 

 

 

True

5

 

 

 

Physiological when RB protein interact with E2F protein and it is pathological when RB interacts with E7?

 

 

 

True

6

 

 

Derangements of the retinoblastoma gene product contribute to disease of only the ocular system, where the result is a childhood eye cancer?

 

 

 

False — you can have problems with kidney.

7

 

 

 

Summary of Primary Paper on Papillomavirus and E7

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8

 

 

 

Summary of Primary Paper on Papillomavirus and E7 Continued

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9

No band is seen in the lysate called RBL and the reason for this is:

Those cells were not incubated with anti-RB antibody

Those cells do not express Rb due to Rb gene mutations. 

This cells do not express RB due to an over expressed transcription repressor protein

None of the above

Q image thumb

 

 

Those cells do not express Rb due to Rb gene mutations. — tumors don’t have RB proteins because RB is a tumor suppressor. 

10

Goal of coimmuno-precipitation studies commonly make the use of an antibody agains only one of the multiple proteins present in a sample, the goal usually being?

To detect whether protein-protiene interactions are present. 

To demonstrate that the protein is recognized by the antibody. 

 

 

 

To detect whether protein-protiene interactions are present. 

11

Middle lane corresponds with a cell lysate that was incubated with an anti-T antibody and this lane functions in the experiment as a:

Negative Control

Positive Control

Both of the Above

None of the Above

 

 

 

Negative Control

12

The reason they could see informative bands with this technique is because of?

Pigment deposition using an enzyme conjugated antibody against an anti-RB antibody.

Pigment deposition using an enzyme conjugated directly to an anti-RB antibody

pigment deposition using an enzyme conjugated directly to an anti-E7 & antibody

None of the above 

 

 

None of the above — these were radio labeled 

13

Q image thumb

 

 

RB prevents E2F from acting as an activator and enhancing transcription. 

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14

 

 

 

Summary of Additional Paper on the Expression Profile of p53

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15

Q image thumb

 

 

 

Tetramer

16

 

 

 

What proccess is not a response to DNA Damage?

 

 

 

Quiescence is not a response to DNA damage. 

17

 

 

 

What is the most commonly mutated gene in cancer?

 

 

 

p53

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