CILS Molecular Techniques Pt. II Flashcards Preview

Fundamentals of Molecular Medicine > CILS Molecular Techniques Pt. II > Flashcards

Flashcards in CILS Molecular Techniques Pt. II Deck (17)
Loading flashcards...
1
Q

True or False?

If a researcher wants to determine if a genes is expressed in cells in a tissue section, the quesiont can be fully answered using either immunohistochemisitry or in in situ hybridization?

A

False — You need to know whether not the protein is present because the mRNA could be present but proteins not made

2
Q

True or False?

Infections of virus and bacteria increase risk for cancer?

A

True

3
Q

The RB protein acts as a brake stopping uncontrolled progression?

A

True

4
Q

RB protein is both a co-repressor and a tumor supressor?

A

True

5
Q

Physiological when RB protein interact with E2F protein and it is pathological when RB interacts with E7?

A

True

6
Q

Derangements of the retinoblastoma gene product contribute to disease of only the ocular system, where the result is a childhood eye cancer?

A

False — you can have problems with kidney.

7
Q

Summary of Primary Paper on Papillomavirus and E7

A
8
Q

Summary of Primary Paper on Papillomavirus and E7 Continued

A
9
Q

No band is seen in the lysate called RBL and the reason for this is:

Those cells were not incubated with anti-RB antibody

Those cells do not express Rb due to Rb gene mutations.

This cells do not express RB due to an over expressed transcription repressor protein

None of the above

A

Those cells do not express Rb due to Rb gene mutations. — tumors don’t have RB proteins because RB is a tumor suppressor.

10
Q

Goal of coimmuno-precipitation studies commonly make the use of an antibody agains only one of the multiple proteins present in a sample, the goal usually being?

To detect whether protein-protiene interactions are present.

To demonstrate that the protein is recognized by the antibody.

A

To detect whether protein-protiene interactions are present.

11
Q

Middle lane corresponds with a cell lysate that was incubated with an anti-T antibody and this lane functions in the experiment as a:

Negative Control

Positive Control

Both of the Above

None of the Above

A

Negative Control

12
Q

The reason they could see informative bands with this technique is because of?

Pigment deposition using an enzyme conjugated antibody against an anti-RB antibody.

Pigment deposition using an enzyme conjugated directly to an anti-RB antibody

pigment deposition using an enzyme conjugated directly to an anti-E7 & antibody

None of the above

A

None of the above — these were radio labeled

13
Q
A

RB prevents E2F from acting as an activator and enhancing transcription.

14
Q

Summary of Additional Paper on the Expression Profile of p53

A
15
Q
A

Tetramer

16
Q

What proccess is not a response to DNA Damage?

A

Quiescence is not a response to DNA damage.

17
Q

What is the most commonly mutated gene in cancer?

A

p53

Decks in Fundamentals of Molecular Medicine Class (77):