Generation of Diversity Flashcards Preview

Fundamentals of Molecular Medicine > Generation of Diversity > Flashcards

Flashcards in Generation of Diversity Deck (37):
1

 

 


 

2

 

 

 

Clonal Selection 

3

 

 

 

How many gene families are there?

4

 

 

 

How are V-Regions different in assembly than Constant Regions?

How are different regions coded for? 

5

 

 

 

Light Chain Gene Rearrangements

6

 

Stepwise Light Chain Rearrangements:

Germline DNA

Rearranged DNA
Primary RNA Transcript

mRNA

PolyPeptide Chain

7

 

 

 

Heavy Chain Rearrangments

8

 

Stepwise Heavy Chain Rearrangements:

Germline DNA

Rearranged DNA
Primary RNA Transcript

mRNA

PolyPeptide Chain

 

9

 

 

 

Heavy Chain Locus

10

 

 

 

Kappa Light Chain Locus

11

 

 

 

Lamda Light Chain Locus

 

 

 

12

 

 

 

Heavy Chain Rearrangements in two different B-Cell Clones

13

 

 

 

Immunoglobulin Heavy and Light Chain Loci

14

 

 

 

Random Recombination of Gene Segments

15

 

 

 

Junctional Diversity 

16

 

 

 

Schematic of V-J Recombination 

17

 

 

 

Junctional Diversity 

18

 

 

 

Potential Repitoire

19

 

 

 

Diversity of CDR's

20

 

 

 

Allelic Exclusion 

21

 

 

Southern Blott

22

 

 

 

PCR Primers

23

 

 

 

Characterization of Junctional Genes

24

 

 

 

When Does Somatic Hypermutation Occur?

25

 

 

 

Somatic Hypermutation 

26

 

 

 

AID

27

 

 

 

AID and the Process of Somatic Hypermutation 

28

 

 

 

Somatic Hypermutation and Secondary Response

29

 

 

 

T-Cell Origin

30

 

 

 

T-Cell Receptor vs. B-Cell

31

 

 

 

Classes of T-Cell

32

 

 

 

TCR Locus

33

 

 

 

Recombination in T-Cells

34

 

 

 

RAG Deficiency 

35

 

 

 

TCR Junctional Diversitu

36

 

 

 

TCell Repitoire

37

 

 

 Light Chain DNA Gene Rearrangement

(Only heavy chains undergo class switching) B is the answer

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