Clonal Selection
How many gene families are there?
How are V-Regions different in assembly than Constant Regions?
How are different regions coded for?
Light Chain Gene Rearrangements
Stepwise Light Chain Rearrangements:
Germline DNA
Rearranged DNA
Primary RNA Transcript
mRNA
PolyPeptide Chain
Heavy Chain Rearrangments
Stepwise Heavy Chain Rearrangements:
Germline DNA
Rearranged DNA
Primary RNA Transcript
mRNA
PolyPeptide Chain
Heavy Chain Locus
Kappa Light Chain Locus
Lamda Light Chain Locus
Heavy Chain Rearrangements in two different B-Cell Clones
Immunoglobulin Heavy and Light Chain Loci
Random Recombination of Gene Segments
Junctional Diversity
Schematic of V-J Recombination
Junctional Diversity
Potential Repitoire
Diversity of CDR’s
Allelic Exclusion
Southern Blott
PCR Primers
Characterization of Junctional Genes
When Does Somatic Hypermutation Occur?
Somatic Hypermutation
AID
AID and the Process of Somatic Hypermutation
Somatic Hypermutation and Secondary Response
T-Cell Origin
T-Cell Receptor vs. B-Cell
Classes of T-Cell
TCR Locus
Recombination in T-Cells
RAG Deficiency
TCR Junctional Diversitu
TCell Repitoire
Light Chain DNA Gene Rearrangement
(Only heavy chains undergo class switching) B is the answer