Introduction to Microscopy

Question 1

Answer 1

Blue binds to negatively charged nucleic acids

Pale signifies, euchromatin that theres a lot of expression going on

S is heterochromatic nucleus with small cytoplasm — relatively inactive.

H and E stain

DNA/RNA is blue

Proteins/Amino acids are pink

Hepatocytes

Question 2

Answer 2

Artifacts

large lipid droplets

All the components of the cell get pushed off to the periphery. Nucleus, golgi, gets pushed off to the side.

Question 3

Answer 3

Artifacts

small lipid droplets

Question 4

Answer 4

2-8

Question 5

Answer 5

Answer #3 : 12-18

You are looking at the inside of a vessel and the vessel wall.

Some cells look like they don’t have a nucleus because the cut is such that the nucleus is not included.

Question 6

Answer 6

Question 7

Answer 7

Question 8

Answer 8

PAS is electric purple

H&E is a dull purple

Tall columnar cells

Question 9

What is Metachromasia

Answer 9

Question 10

Increasing Magnification also…

Answer 10

Question 11

Can you increase magnification without increasing resolution?

Answer 11

Yes, digitally

Question 12

What is TEM?

Answer 12

Question 13

Light Microscope vs. TEM

Answer 13

Question 14

What is an SEM

Answer 14

Question 15

What type of microscopy?

Answer 15

Scanning Electron Microscope

Question 16

What type of microscopy and what is it scanning

Answer 16

Intestinal Epithelium on an SEM

Question 17

Answer 17

Question 18

Lipid Bilayer

Answer 18

Question 19

Answer 19

O2 and H2O and Steroids

Charged will have difficult time getting through

Just a little water can pass through (without aquaporins)

Amino acids, glucose, Chloride, none of them can pass through.

Question 20

Lipids in the bilayer

Answer 20

Question 21

Proteins in the Lipid Bilayer

Answer 21

Question 22

How can we attach proteins to the membrane?

Answer 22

Question 23

6 Functions/Types of Membrane Proteins

Answer 23

Question 24

Example of Structural Protein

Answer 24

Question 25

Example of Enzymes in the membrane

Answer 25

Question 26

Examples of Linkers in the Membrane

Answer 26

Question 27

Examples of Recpetors

Answer 27

Question 28

Example of protein channels in the membrane

Answer 28

Question 29

Example of Protein Pumps in the Membrane

Answer 29

Question 30

Movement of Molecules through the membrane

Answer 30

Question 31

Facilitated Diffusion vs. Active Diffusion

Answer 31

Question 32

Answer 32

E — peripheral proteins are only attached to membrane via charged interactions. Ionic detergents disrupt the membrane.

Question 33

Carbohydrates in the lipid bilayer

Answer 33

Question 34

Blood, Carbohydrates, and the membrane

Answer 34

Question 35

Lipid Rafts

Answer 35

Question 36

Answer 36