Blue binds to negatively charged nucleic acids
Pale signifies, euchromatin that theres a lot of expression going on
S is heterochromatic nucleus with small cytoplasm — relatively inactive.
H and E stain
DNA/RNA is blue
Proteins/Amino acids are pink
Hepatocytes
Artifacts
large lipid droplets
All the components of the cell get pushed off to the periphery. Nucleus, golgi, gets pushed off to the side.
Artifacts
small lipid droplets
2-8
Answer #3 : 12-18
You are looking at the inside of a vessel and the vessel wall.
Some cells look like they don’t have a nucleus because the cut is such that the nucleus is not included.
PAS is electric purple
H&E is a dull purple
Tall columnar cells
What is Metachromasia
Increasing Magnification also…
Can you increase magnification without increasing resolution?
Yes, digitally
What is TEM?
Light Microscope vs. TEM
What is an SEM
What type of microscopy?
Scanning Electron Microscope
What type of microscopy and what is it scanning
Intestinal Epithelium on an SEM
Lipid Bilayer
O2 and H2O and Steroids
Charged will have difficult time getting through
Just a little water can pass through (without aquaporins)
Amino acids, glucose, Chloride, none of them can pass through.
Lipids in the bilayer
Proteins in the Lipid Bilayer
How can we attach proteins to the membrane?
6 Functions/Types of Membrane Proteins
Example of Structural Protein
Example of Enzymes in the membrane
Examples of Linkers in the Membrane
Examples of Recpetors
Example of protein channels in the membrane
Example of Protein Pumps in the Membrane
Movement of Molecules through the membrane
Facilitated Diffusion vs. Active Diffusion
E — peripheral proteins are only attached to membrane via charged interactions.
Ionic detergents disrupt the membrane.
Carbohydrates in the lipid bilayer
Blood, Carbohydrates, and the membrane
Lipid Rafts