Introduction to Microscopy Flashcards Preview

Fundamentals of Molecular Medicine > Introduction to Microscopy > Flashcards

Flashcards in Introduction to Microscopy Deck (36):
1

Blue binds to negatively charged nucleic acids 

Pale signifies, euchromatin that theres a lot of expression going on 

S is heterochromatic nucleus with small cytoplasm — relatively inactive. 

H and E stain

DNA/RNA is blue 

Proteins/Amino acids are pink

Hepatocytes

 

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Artifacts

large lipid droplets

All the components of the cell get pushed off to the periphery. Nucleus, golgi, gets pushed off to the side. 

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Artifacts

small lipid droplets

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2-8

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Answer #3 : 12-18

You are looking at the inside of a vessel and the vessel wall. 

Some cells look like they don’t have a nucleus because the cut is such that the nucleus is not included. 

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PAS is electric purple 

H&E is a dull purple

Tall columnar cells

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What is Metachromasia

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Increasing Magnification also...

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Can you increase magnification without increasing resolution?

 

 

Yes, digitally 

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What is TEM?

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Light Microscope vs. TEM

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What is an SEM 

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What type of microscopy?

 

 

 

Scanning Electron Microscope

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What type of microscopy and what is it scanning

 

 

 

Intestinal Epithelium on an SEM

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Lipid Bilayer

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O2 and H2O and Steroids

Charged will have difficult time getting through

Just a little water can pass through (without aquaporins)

Amino acids, glucose, Chloride, none of them can pass through.

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Lipids in the bilayer

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Proteins in the Lipid Bilayer

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How can we attach proteins to the membrane?

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6 Functions/Types of Membrane Proteins

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Example of Structural Protein

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Example of Enzymes in the membrane

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Examples of Linkers in the Membrane

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Examples of Recpetors

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Example of protein channels in the membrane

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Example of Protein Pumps in the Membrane 

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Movement of Molecules through the membrane

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Facilitated Diffusion vs. Active Diffusion 

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E — peripheral proteins are only attached to membrane via charged interactions.

Ionic detergents disrupt the membrane.

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Carbohydrates in the lipid bilayer

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Blood, Carbohydrates, and the membrane

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Lipid Rafts

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