Mitochondria and Fluorescent Microscopy Flashcards Preview

Fundamentals of Molecular Medicine > Mitochondria and Fluorescent Microscopy > Flashcards

Flashcards in Mitochondria and Fluorescent Microscopy Deck (40):
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Overview and Review

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What type of Microscopy?

Indicate main feature of mitochondria.

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Left — Condensed configuration — High ATP production 

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Right — Shelf like cristae — orthodox configuration = low ATP 

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Autoradiography

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What are reporter genes?

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Proteins and Reporter Genes

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GFP

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Fluorescence in Embryonic Development

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Flow Cytometry 

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Flow Cytometry Cont. 

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Even More on Flow Cytometry

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Immunofluorescence and Microscopy 

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Staining in Immunofluorescence 

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What type of treatment to tissue?

 

 

 

Immunofluorescence

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Indirect Immunofluorescnece 

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Confocal Microscopy 

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Immunohistochemistry 

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Immunohistochemistry Cont. 

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What technique was used?

 

 

 

Immunohistochemsitry

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Basic Immunohistochemsitry

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CISH

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Tissue Treatment?

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CISH Continued

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CISH Pt. 500000

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Histochemical Stains

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Enzyme Histochemistry 

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Common Histochemical Stains and Locations

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Laser Capture Microdissection

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Laser Capture Pt. 2

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Slide Scanning

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Common Scenario in Microscopic Analysis 

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