Overview and Review
What type of Microscopy?
Indicate main feature of mitochondria.
Left — Condensed configuration — High ATP production
Right — Shelf like cristae — orthodox configuration = low ATP
Autoradiography
What are reporter genes?
Proteins and Reporter Genes
GFP
Fluorescence in Embryonic Development
Flow Cytometry
Flow Cytometry Cont.
Even More on Flow Cytometry
Immunofluorescence and Microscopy
Staining in Immunofluorescence
What type of treatment to tissue?
Immunofluorescence
Indirect Immunofluorescnece
Confocal Microscopy
Immunohistochemistry
Immunohistochemistry Cont.
What technique was used?
Immunohistochemsitry
Basic Immunohistochemsitry
CISH
Tissue Treatment?
CISH Continued
CISH Pt. 500000
Histochemical Stains
Enzyme Histochemistry
Common Histochemical Stains and Locations
Laser Capture Microdissection
Laser Capture Pt. 2
Slide Scanning
Common Scenario in Microscopic Analysis