Mitochondria and Fluorescent Microscopy Flashcards
(40 cards)
1
Q
Overview and Review
A
2
Q
What type of Microscopy?
Indicate main feature of mitochondria.
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3
Q
A
Left — Condensed configuration — High ATP production
4
Q
A
Right — Shelf like cristae — orthodox configuration = low ATP
5
Q
A
6
Q
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7
Q
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8
Q
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9
Q
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10
Q
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11
Q
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12
Q
Autoradiography
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13
Q
What are reporter genes?
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14
Q
Proteins and Reporter Genes
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15
Q
A
16
Q
GFP
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17
Q
Fluorescence in Embryonic Development
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18
Q
Flow Cytometry
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19
Q
Flow Cytometry Cont.
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20
Q
Even More on Flow Cytometry
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21
Q
Immunofluorescence and Microscopy
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22
Q
Staining in Immunofluorescence
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23
Q
What type of treatment to tissue?
A
Immunofluorescence
24
Q
Indirect Immunofluorescnece
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25
Confocal Microscopy
26
Immunohistochemistry
27
Immunohistochemistry Cont.
28
What technique was used?
Immunohistochemsitry
29
Basic Immunohistochemsitry
30
CISH
31
Tissue Treatment?
32
CISH Continued
33
CISH Pt. 500000
34
Histochemical Stains
35
Enzyme Histochemistry
36
Common Histochemical Stains and Locations
37
Laser Capture Microdissection
38
Laser Capture Pt. 2
39
Slide Scanning
40
Common Scenario in Microscopic Analysis
