Effector Functions of Ig Classes
Most Abundant = IgG = workhorseIgD and IgE are rarest.
Immunity to fetus — IgG
IgM always expressed first
IgG expressed during memory response Mucosa is IgA
[Antibody] vs. Time After Immunization
IgM vs. IgG after First Exposure
IgG
Opsonization = IgG
Complement = IgM and IgG
Hypersensitivity = IgE FC Receptors = Mass Cells and Basophils express them
IgD
IgA = Respiratory tract
Anaphylaxis = IgE
IgG Convalescent patient
IgA is Breast feeding
Complement = IgG
Process of Opsonization?
Ig Class?
What Ig are involved in Opsonization?
Fraction of Adult Level of Serum Immunoglobulins
Affinity of IgM vs. IgG
Physiological Roles of Ag-Ab Interaction
Neutralization
FC Receptors
IgE Role in Allergies
Immediate Hypersensitivity
Antibody Isotype and Effector Functions
Affinity vs. Avidity
IgM vs. IgG Affinity vs. Avidity
Ag-Ab Complex Formation
Antibody Titer
What is the Antibody Titer?
This is a titer for 1:512
Elisa
Elisa Assay. You’re looking for antibody against organisms.
You coat the well with HIV protein.
Add Test Antiserum
Western Blot
Isohemagluttinins
Blood Typing
Rh Disease
Coombs Test
Indirect Coombs Test
Direct vs. Indirect Coombs Test
Coombs Reagent
and
RhoGAM
Isohemagglutinins are IgM class which doesn’t cross the placental barrier so you don’t have those types of problems.