Intro to Microscopy Flashcards by Omar Saqr

Q

Describe how a light microscope works?

A

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Q

Knurled Head Locking Screw

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Q

The Specimen Holder/Stage

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Q

Light Intensity Control Knob

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Q

Abbe Condenser

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Q

Revolving Nose Pieces and Objective Lenses

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Q

Widefield Eyepiece

Interpupillary Scale

A

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Q

Course Focus and Fine Focus Knob

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Q

Knurled Diopeter Ring

A

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Q

Knobs Controlling the Mechanical Stage

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Q

Identify Basic Features of Cells and Tissues

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Q

Cytoplasm vs. Cytosol

A

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Q

Identify the Tissue, The Microscopy, the outlined green, and the blue arrows

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Q

Approximate the number of cells.

Identify the Blue Arrows?

Green Outline?

A

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Q

Identify the green brackets

Identify the yellow Arrows

What type of microscopy?

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Q

What are the 4 major steps of slide preparation?

A

Q

Explain the Process of Tissue Fixation

A

Q

Explain Chemical Fixation

A

Q

Explain the process of Embedding

A

Q

Explain the process of sectionig

A

Q

Explain the Process of Staining

A

Q

Hematoxylin vs. Eosin

A

Q

What type of stain is this?

What type of Tissue?

Nucleus vs. Proteins in staining

A

Q

What type of staining is this?

Explain Cytoplasmic Basophillia.

A

What type of stain? What are the green arrows?

What type of stain? What comprises the outlined the region and why?

What type of stain? Why the color?

What type of stain? Identify heterochromatin and Euchromatin. Red Arrow

Explain Periodic and Acid Schiff

What type of stain? What gets stained?

Identify the clear protions of these stains Describe what they are generally and point to them specifically.

Identify The black Arrow? The Blue Arrow? Describe the pink space between.

Shrinkage and Artifacts

What type of Stain? Green Arrows?

What type of stain? Identify the Blue and Black Arrows.