Practice Problems Flashcards Preview

Fundamentals of Molecular Medicine > Practice Problems > Flashcards

Flashcards in Practice Problems Deck (31):
1

 

 

 

The Chirality of Which Carbon will determine the L or D configuration in an amino acid?

 

 

 

Alpha carbon (Adjacent to the Carbonyl)

2

 

 

 

Digestive problems when eating milk means

 

 

Difficulty splitting a Glycosidic Bond

NOT 

a Sugar Bond (No such thing)

3

 

 

 

Northern Blot vs. Southern Blot

 

 

Northern Blot = RNA
Southern Blot = DNA


Which means adding NaOH to a northern blot will hydrolyze the nucleic acid (RNA) because of a 2' hydroxy group on the RNA not present on the DNA

4

 

 

Inability to alter this position in the purine ring will lead to Adenosine Deaminase Deficiency 

 

 

 

Amines are on the 6th position of the purine ring.

5

 

 

 

The flip-flop of phosphatidylserine between membrane leaflets exposes which part of the phosphatidylserine to the environment?

 

 

 

The head group because it is hydrophillic and the enviorment is polar

6

 

 

 

What type of bonds allow nucleotides to form long polymers?

 

 

 

A phosphodiester bond (no adjacent carbonyle on it)

7

 

 

 

Mitochondrial Diseases 

 

 

 

Inherited from mom and is dominant because the father doesn't have a mitochondrial genome. 

8

 

 

 

What is the optimal pH for Lysozymes?

 

 

 

pH between 4-6

9

 

 

 

How do you get protons into the lysosome?

 

 

 

Active pumping of protons into the organelle.

10

 

 

 

Exhaling CO2 will do what to the pH of blood

 

 

Exhaling CO2 will cause the equilibrium to shift towards CO2 and leave less free protons increasing pH (less acidic)

11

 

 

What amino acid side chain albumin participates in the buffering effct of keeping blood pH between 7.2 and 7.4?

 

 

 

Histidine

12

 

 

 

Formation of Phospholipids do what to entropy of water?

 

 

 

Increases entropy of water

13

 

 

 

Gout is the acummulation of what in the foot?

 

 

 

 

Crystals which are due to accumulation of purines. 

14

 

 

A single stranded DNA Molecule contains 20% A, 25% T, 30 % G, 25% . When the complement of this strand is synthesized, the T content of the resulting duplex will be what percentage?

 

 

 

 

22.5%

15

 

 

 

How is HbA1C different from Hemeglobin?

 

 

It is a glycosylated version of Hemeglobin.

N-Terminal valine is glycosylated in the beta chains. 

16

 

 

 

How can you make a drug more soluble?

 

 

 

 

Add a hydrophillic group needs to be added. 

17

 

 

 

How do cell lines complement each other?

 

 

 

If mutations are at different genes?

18

 

 

 

Exonuclease activity of DNA Polymerase is always

 

 

 

3' to 5' exonuclease activity

19

 

 

 

Fragile X Syndrome

 

 

 

An extended triplet nucleotide repeat

20

 

 

 

Xeroderma Pigmentosum is what type of mutation?

 

 

 

Nucleotide Excision Repair Mutation

21

 

 

 

Which nucleotide base have amine groups to deaminate?

 

 

 

A, G and C

Adenosine, Guanine, and Cytosine

(Not Uracil, Thymine)

22

 

 

 

High mutation rate of HIV is due to what enzyme?

 

RNA Polymerase

 

When RNA reverse transcribes DNA it doesn't have any proofreading mechanisms so more mutations occur. 

23

 

 

 

Standard notation for writting DNA Sequences is to write them in what order?

 

 

 

5' to 3' 

24

 

 

 

Prader Willi Syndrome is what type of Mutation?

 

 

 

Deletion on the long arm of Chromosome 15

25

 

 

 

Bloom Syndrome

 

 

 

Defect in DNA Helicase

 

26

 

 

 

Levoflaxin/Ciproflaxin inhibits which part of the bacterial enzyme... 

 

 

 

Bacterial Topoisomerase

27

 

 

 

Cokayne Syndrome

 

 

 

Defect in transcription coupled DNA Pair

28

 

 

 

Lack of P53 Activity leads to 

 

 

 

increase in DNA mutation rates because the checkpoint is no longer available. 

29

 

 

 

Nascent Okazaki Fragments have uracil why?

 

 

 

Because they have primers in them. 

30

 

 

 

Philidelphia Chromosome 

 

 

 

Translocation between 9 and 22

Creation of fusion protein not normally found in cells

31

 

 

 

DNA Synthesis requires the following components

 

 

Intact DNA

Replisome Complex

Deoxyribonucleoside triphosphates

Ribonucleoside Triphosphates

Decks in Fundamentals of Molecular Medicine Class (77):