What's the difference between an GTP-ase and an Exchange Factor
Ase— suffix means it hydrolyzes and GTP to GDP or ATP to ADP
Exchange Factor — Not a hydrolysis reaction but an exchange — it switches them out.
Quickly draw an image with all the following features:
What is Chromatin?
Size and number of folds of DNA?
Definition of Chromatin
What are the histone Proteins?
Features of DNA
Derived from an extended array of nucleosomes
Chromatin Higher Order Structure
Euchromatin Vs. Heterochromatin
Reading the Histone Code
Genetics vs. Epigenetics
Genetics — DNA Sequence
Epigenetic’s — Histone Modifications — Actually be inherited — Tells you what histones to acetylate/Methylate and therefore what genes to activate and deactivate.
Function and Overview of the Cell Cycle
Steps in the Mitotic Cell Cycle
Cell Cycle Check Points
Regulation of Cell Cycle Progression
G1 - S Transition Regulation
Without RB you lose G1 to S checkpoint so it goes from G1 to S freely and a lot of genetic mistakes/mutations can occur.
Lose RB means you lose the suppression of tumor formation.
If you have a mutation of NES means too much Cyclin D in the nucleus then phosphorylates proteins too early in G1 and sometimes in s-phase and drives the cycle forward prematurely.
CDK/Cyclin regulation of S-Phase
Cyclin/CDK regulation of G2 to M to G1
Mitotic CDK/Cyclin — Cyclin B
Cyclin B levels rise then complexes with CDK
CDK phosphorylates 100’s of proteins.
Lamina Proteins depolymerize
Completion of Cytokinesis
Difference between Meiosis and Mitosis
Meiosis has two cell divisions not just one. Because homologous choromosomes in meiosis they line up on the same equatorial plane they undergo homologous recombination.
What is a Karyotype
Steps for creating a Karyotype
Normal vs. Altered Karyotype
What can you infer from this image?
What is FISH?
How does FISH Work?
Chromosome Painting/Spectral Karyotyping
What are we Looking for in Chromosome Painting
Looking for loss or scrambling of chromosomes.
Very common in certain types of cancer to have scrambled karyotypes.
What disease is associated with this Karyotype?