Flashcards in Biochem Lab Color Rxns Deck (30):
1
purpose of biuret test
detect presence of peptide bonds
2
purpose of ninhydrin
presence of alpha AA
3
purpose of xanthoproteic
presence of aromatic AA
4
purpose of millon's
presence of tyrosine (phenolic group)
5
purpose of hopkins-cole
presence of indole group of tryptophan
6
purpose of sakaguchi
un or monosubstituted guanidine in arginine
7
purpose of nitroprusside
presence of cysteine
8
purpose of fohl's
presence of sulfur-containing aa
9
purpose of test for amides
R groups of glutamine and arginine
10
purpose of pauly
test for tyrosine and histidine
11
(+) result of biuret
violet solution
12
(+) result of ninhydrin
blue-violet sol'n
13
(+) result of xanthoproteic
yellow ppt/soln
14
(+) result of millon's
red/reddish-brown solution
15
(+) result of hopkins-cole
purple ring at junction where 2 liquids meet
16
(+) result of sakaguchi
red coloration
17
(+) result of nitroprusside
red soln
18
(+) result of fohl's
black or brown sediment
19
(+) result of test for amides
red litmus to blue
evolution of gas
20
(+) result of pauly test
red coloration
21
reagents used in biuret
2.5 M NaOH
0.1 M CuSO4
22
reagents used in ninhydrin
0.1% ninhydrin solution
23
reagents used in xanthoproteic
Conc. HNO3
Conc. NaOH
24
reagents used in millon's
Millon's reagent
25
reagents used in hopkins-cole
Hopkins Cole reagent
H2SO4
26
reagents used in sakaguchi
10% NaOH
0.02% Naphthol solution
2% NaOBr
27
reagents used in nitroprusside
3M NaOH
2% Nitroprusside
28
reagents used in fohl's
30% NaOH
5% (CH3COO)2Pb
29
reagents used in test for amides
20% NaOH
30