Biochem Lab Color Rxns Flashcards by Carmina Mislang

Q

purpose of biuret test

A

detect presence of peptide bonds

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

purpose of ninhydrin

A

presence of alpha AA

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

purpose of xanthoproteic

A

presence of aromatic AA

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

purpose of millon’s

A

presence of tyrosine (phenolic group)

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

purpose of hopkins-cole

A

presence of indole group of tryptophan

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

purpose of sakaguchi

A

un or monosubstituted guanidine in arginine

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

purpose of nitroprusside

A

presence of cysteine

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

purpose of fohl’s

A

presence of sulfur-containing aa

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

purpose of test for amides

A

R groups of glutamine and arginine

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

purpose of pauly

A

test for tyrosine and histidine

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

(+) result of biuret

A

violet solution

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

(+) result of ninhydrin

A

blue-violet sol’n

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

(+) result of xanthoproteic

A

yellow ppt/soln

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

(+) result of millon’s

A

red/reddish-brown solution

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

(+) result of hopkins-cole

A

purple ring at junction where 2 liquids meet

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

(+) result of sakaguchi

A

red coloration

Q

(+) result of nitroprusside

A

red soln

Q

(+) result of fohl’s

A

black or brown sediment

Q

(+) result of test for amides

A

red litmus to blue

evolution of gas

Q

(+) result of pauly test

A

red coloration

Q

reagents used in biuret

A

5 M NaOH

0. 1 M CuSO4

Q

reagents used in ninhydrin

A

0.1% ninhydrin solution

Q

reagents used in xanthoproteic

A

Conc. HNO3

Conc. NaOH

Q

reagents used in millon’s

A

Millon’s reagent

reagents used in hopkins-cole

Hopkins Cole reagent | H2SO4

reagents used in sakaguchi

10% NaOH 0.02% Naphthol solution 2% NaOBr

reagents used in nitroprusside

3M NaOH | 2% Nitroprusside

reagents used in fohl's

30% NaOH | 5% (CH3COO)2Pb

reagents used in test for amides

20% NaOH

reagents used in pauly

``` Diazo reagent (1% sulfanilic acid + 5% NaNO2 solution) 10% Na2CO3 ```