GPHT LAB - Adv and Disadv

Question 1

adv

cheap, readily available, easy to prepare, relatively stable

Answer 1

formaldehyde

Question 2

adv

does not overharden tissue

Answer 2

formaldehyde

Question 3

adv

preserves fat and mucin

Answer 3

formaldehyde

Question 4

adv

best fixative for nervous tissue

Answer 4

formaldehyde

Question 5

disadv

irritating to skin causing allergic dermatitis

Answer 5

formaldehyde

Question 6

disadv

fumes irritating to eyes and nostrils causing allergic rhintis and sinusitis

Answer 6

formaldehyde

Question 7

disadv

considerable shrinkage of tissues

Answer 7

formaldehyde

Question 8

disadv

reduction of both eosinophilic and basophilic staining of cells

Answer 8

formaldehyde

Question 9

disadv

forms abundant brown artifacts, pigments, granules

Answer 9

formaldehyde

Question 10

adv

has stable nature, stable effect on tissues, giving firmer texture and color

Answer 10

glutaraldehyde

Question 11

adv

preserves cellular and plasma proteins better

Answer 11

glutaraldehyde

Question 12

adv

excellent fixative for routine paraffins sections

Answer 12

paraformaldehyde

Question 13

adv

used for thin and ultrathin sections for plastic embedding

Answer 13

paraformaldehyde

Question 14

adv

gives better staining of nuclei and connective tissue

Answer 14

Mercuric chloride

Question 15

adv

recommended for renal, fibrin and CT

Answer 15

Mercuric chloride

Question 16

disadv

corrodes metal except nickel

Answer 16

Mercuric chloride

Question 17

adv

strong protein precipitant

Answer 17

chromate fixatives

Question 18

adv

preserves carbohydrates

Answer 18

chromate fixatives

Question 19

disadv

forms a precipitate of insoluble suboxide

Answer 19

chromate fixatives

Question 20

disadv

poor glycogen fixation

Answer 20

chromate fixatives

Question 21

adv

fixes but does not precipitate cytoplasmic contents

Answer 21

potassium dichromate

Question 22

adv

preserves mitochondria

Answer 22

potassium dichromate

Question 23

adv

used mainly for mucopolysaccharides

Answer 23

lead fixatives

Question 24

adv

precipitates protein

Answer 24

lead fixatives

Question 25

adv | best fixative for glycogen

Answer 25

picric acid fixatives

Question 26

adv | fixes and precipitates nucleoproteins

Answer 26

acetic acid fixative

Question 27

adv | precipitates chromosomes and chromatin materials, which makes it useful in nuclear studies

Answer 27

acetic acid fixative

Question 28

disadv | causes swelling of tissues

Answer 28

acetic acid fixative

Question 29

disadv | destroys mitochondria and golgi elements

Answer 29

acetic acid fixative

Question 30

adv | recommended for phosphatases and lipases studies

Answer 30

acetone

Question 31

adv | used for fixing brain tissue for rabies

Answer 31

acetone

Question 32

disadv | evaporates rapidly

Answer 32

acetone

Question 33

adv | ideal for smaller tissue fragments

Answer 33

alcohol fixatives

Question 34

adv | EXCELLENT for glycogen preservation

Answer 34

alcohol fixatives

Question 35

disadv | causes polarization of glycogen

Answer 35

alcohol fixatives

Question 36

adv | fixes conjugate fats and lipids permanently by making them insoluble during subsequent treatment with alcohol and xylene

Answer 36

osmium tetroxide fixatives

Question 37

disadv slow fixing agent suitable for small tissues only

Answer 37

osmium tetroxide fixatives