Flashcards in GPHT LAB - Adv and Disadv Deck (37):
1
adv
cheap, readily available, easy to prepare, relatively stable
formaldehyde
2
adv
does not overharden tissue
formaldehyde
3
adv
preserves fat and mucin
formaldehyde
4
adv
best fixative for nervous tissue
formaldehyde
5
disadv
irritating to skin causing allergic dermatitis
formaldehyde
6
disadv
fumes irritating to eyes and nostrils causing allergic rhintis and sinusitis
formaldehyde
7
disadv
considerable shrinkage of tissues
formaldehyde
8
disadv
reduction of both eosinophilic and basophilic staining of cells
formaldehyde
9
disadv
forms abundant brown artifacts, pigments, granules
formaldehyde
10
adv
has stable nature, stable effect on tissues, giving firmer texture and color
glutaraldehyde
11
adv
preserves cellular and plasma proteins better
glutaraldehyde
12
adv
excellent fixative for routine paraffins sections
paraformaldehyde
13
adv
used for thin and ultrathin sections for plastic embedding
paraformaldehyde
14
adv
gives better staining of nuclei and connective tissue
Mercuric chloride
15
adv
recommended for renal, fibrin and CT
Mercuric chloride
16
disadv
corrodes metal except nickel
Mercuric chloride
17
adv
strong protein precipitant
chromate fixatives
18
adv
preserves carbohydrates
chromate fixatives
19
disadv
forms a precipitate of insoluble suboxide
chromate fixatives
20
disadv
poor glycogen fixation
chromate fixatives
21
adv
fixes but does not precipitate cytoplasmic contents
potassium dichromate
22
adv
preserves mitochondria
potassium dichromate
23
adv
used mainly for mucopolysaccharides
lead fixatives
24
adv
precipitates protein
lead fixatives
25
adv
best fixative for glycogen
picric acid fixatives
26
adv
fixes and precipitates nucleoproteins
acetic acid fixative
27
adv
precipitates chromosomes and chromatin materials, which makes it useful in nuclear studies
acetic acid fixative
28
disadv
causes swelling of tissues
acetic acid fixative
29
disadv
destroys mitochondria and golgi elements
acetic acid fixative
30
adv
recommended for phosphatases and lipases studies
acetone
31
adv
used for fixing brain tissue for rabies
acetone
32
disadv
evaporates rapidly
acetone
33
adv
ideal for smaller tissue fragments
alcohol fixatives
34
adv
EXCELLENT for glycogen preservation
alcohol fixatives
35
disadv
causes polarization of glycogen
alcohol fixatives
36
adv
fixes conjugate fats and lipids permanently by making them insoluble during subsequent treatment with alcohol and xylene
osmium tetroxide fixatives
37