CC Lab - Glucose Flashcards

1
Q

measures blood glucose exactly 2 hours after you start eating a meal

A

Two-hour Post Prandial Blood Sugar

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2
Q

how many carbs (in g) must be eaten for the Two-hour Post Prandial Blood Sugar

A

at least 100g

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3
Q

glycosylated hemoglobin

A

HbA1C

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4
Q

Other names of gluc

A

dextrose and blood sugar

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5
Q

possible tests requested by physician

A
FBS 
RBS
OGTT, OGCT
2 PPBS
HbA1c
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6
Q

test for pregnant women/with gestational diabetes

A

OGTT/OGCT

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7
Q

test which requires NPO for 6-8 hrs

A

FBS

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8
Q

Test for monitoring DM

A

HbA1C

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9
Q

HbA1C test is done every ___

A

3 months

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10
Q

excess glucose is stored in __

A

liver

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11
Q

excess glucose may be stored as (2)

A

glycogen

adipose tissue

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12
Q

hormones that regulate gluc levels

A

insulin and glucagon

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13
Q

a hyperglycemic agent

A

glucagon

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14
Q

primary hypoglycemic agent

A

insulin

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15
Q

methods for glucose determination (3)

A

reduction method
schiff base reaction with orthotoluidine
enzymatic method

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16
Q

experiment proper (name??)

A

enzymatic colorimetric glucose testing

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17
Q

most important carbohydrate

18
Q

CC which needs EDTA tube

19
Q

the rest of CC tests need (Color of top)

A

red or yellow top

20
Q

Principle in glucose testing (?)

A

glucose + oxygen + water ——> gluconic acid + H2O2

see notebook

21
Q

insulin is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis

A

gluconeogenesis

22
Q

glucagon is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis

A

glycogenolysis

23
Q

3 methods for glucose determination

A

reduction method

schiff base reaction with orthotoluidine

24
Q

3 methods for glucose determination
alkaline method
based on the ability of glucose to reduce copper or ferricyanide

A

reduction method

25
type of reduction method which includes Nelson-Somogyi, Folin-Wu, Iodometric
Copper reduction
26
type of reduction method | inverse colorimetry
Ferricyanide method
27
principle in ferricyanide method
ferricyanide (yellow) is converted to ferrocyanide (colorless)
28
3 methods for glucose determination | in a hot acidic medium or glacial acetic acid
schiff base reaction with orthotoluidine
29
Purpose of glucose measurement
Determine capacity of body to maintain normal level of glucose
29
Purpose of glucose measurement
Determine capacity of body to maintain normal level of glucose
30
glucose measurement significant in diagnosing what disease
Diabetes mellitus
31
Indicator in the glucose measurement
Quinoneimine dye
32
Color of quinoneimine dye
Red violet
33
Assay requirements
Wavelength - 500 nm Optical path - 1 cm Temperature - 20-25C Measurement - against reagent blank
34
Only --- reagent blank used per series
1
35
How long to incubate and at what temp
10 min | 20-25
36
How much standard solution must be placed
0.01 ml
37
How much control normal serum, control pathologic serum, patient's serum and standard solution must be placed
0.01 ml
38
Total volume in the cuvets
1.01 ml except for the blank which is 1.0 ml only
39
Pipetting scheme
Semi-micro