CC LEC 2 - Enzymes II Flashcards Preview

Botany > CC LEC 2 - Enzymes II > Flashcards

Flashcards in CC LEC 2 - Enzymes II Deck (63)
Loading flashcards...
1


 128,000 Daltons
 Interconversion of lactic acid and
pyruvic acid

LD - Lactate Dehydrogenase

2

Atypical LD
LD-6: ??

alcohol dehydrogenase

3

Atypical LD
Migrates cathodal to LD-5
 Present in patients with
arteriosclerotic cardiovascular failure
 Grave prognosis, signifies impending
death

alcohol dehydrogenase

4

Atypical LD
LD complexed with IgA or IgG, migrates between LD-3 and LD-4

Macro-LD:

5

– to measure LD-1(HHHH) because H-units have great affinity

*a-Hydroxybutyrate dehydrogenase activity

6

Methods for LD
forward reaction
- mixture of phenazine methosulfate
and nitroblue
- tetrazolium reacts with NADH
producing blue purple color
- pH8.3–8.9

1. Wacker method

7

Methods for LD
– reverse
reaction
- NADH is cosubstrate, consumed
during reaction

- pH7.1–7.6

Wroblewski La Due method

8

Methods for LD
- 3 times faster, smaller sample, shorter
reaction time
- Susceptible to substrate exhaustion
and loss of linearity

Wroblewski La Due method

9


 Transfer of amino group between aspartic acid and a-ketoacids

Aspartate Aminotransferase

10

ast coenz

Pyridoxal phosphate is coenzyme

11

ast isoenz
predominant form in serum

Cytoplasmic Isoenzyme –

12

ast isoenz
increased during cell necrosis

Mitochondrial Isoenzyme

13

AST Method
– coupled enzymatic reaction
- Malate dehydrogenase is indicator enzyme
- Decrease in absorbance at 340nm
- Optimal pH 7.3-7.8

Karmen method

14

AST Method
– ketoacids react with 2,4 – DNPH to form ketoacid hydrazines
- Product is intense brown color measured at 505nm

Reitman-Frankel Method

15


- Catalyzes transfer of amino groups from alanine to a-ketoglutarate

Alanine Aminotransferase

16

Liver specific enzyme

Alanine Aminotransferase

17

Not elevated in heart disease unless accompanied with liver disease

Alanine Aminotransferase

18

Tends to be higher and lasts longer than AST in acute inflammatory conditions of the liver

Alanine Aminotransferase

19

Requires Mg2+ and Mn2+ as activators

Alkaline Phosphatase

20

class 3 enz

ALP

21

Hydrolysis of various phosphomonoesters; making it nonspecific in alk

ALP

22

ALP isoenz Increased in B and O secretors

Intestinal ALP

23

Abnormal ALP isoenz

Regan
Nagao

24

Abnormal ALP
Variant of Regan
- Detected in metastatic carcinoma of pleural surfaces and in adenocarcinoma of pancreas and bile duct

nagao

25

Abnormal ALP
Highest in ovarian and gynecologic cancers
- Used for monitoring treatment because it disappears upon successful therapy

regan

26

ALP ISOENZ (3) are most heat stable and resist denaturation at 65C for 30 minutes

Placental, Regan, and Nagao

27

inhibits intestinal (75%) and
placental (80%) ALP ISOENZ

Phenylalanine

28

Nagao is also inhibited by __

L-leucine

29

inactivates bone(90%0 more
than liver(60%) ALP

Urea(2M)

30

Hydrolysis of various phosphomonoesters at acidic pH (5.0)

ACP

Decks in Botany Class (324):