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Flashcards in GPHT LAB - Clearing Deck (71)
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1

in dealcoholization, tissue section is immersed in __

ante-medium

2

raises the refractive index of the tissue

ante-medium

3

clearing agents must be miscible with both __

alcohol and paraffin wax

4

does not cause to much brittleness

benzene

5

best routine clearing agent

benzene

6

can cause aplastic anemia

benzene

7

used for large, thick, hard tissues

chloroform

8

slow acting
toxic to liver
does not make tissues translucent
tissues tend to float

chloroform

9

used for cleaning knives

xylene

10

diluent in mounting medium

xylene

11

most common clearing agent

xylene

12

for tissues less than 5 mm

xylene

13

useful for paraffin embedding

xylene

14

becomes milky/turbid if water is still present (incomplete dehydration)

xylene

15

overclearing causes tissue to become

hard and brittle

16

sub for xylene

toluene

17

subs for xylene/benzene

toluene

18

for CNS, skin, smooth muscle, cytological work

cedar oil/cedar wood oil

19

can leave tissue for a very long time w/o dehydration

cedar oil/cedar wood oil

20

penetrates well = best clearing agent

cedar oil/cedar wood oil

21

expensive and turbid

cedar oil/cedar wood oil

22

SLOW
Makes tissue brittle
can be faked

clove oil

23

prevents clove oil from becoming brittle

oil of bergamot

24

does not extract aniline dyes

oil of bergamot

25

can be used with celloidin prep

oil of bergamot

26

spanish hop oil or thyme oil or mix

oil of origanum

27

dissolves out aniline dyes

oil of origanum

28

does not dissolve celloidin

oil of origanum

29

other names of oil of wintergreen

methyl salicylate, artificial oil

30

for delicate tissues bc hardening effect of alcohol can be avoided

oil of wintergreen

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