Flashcards in GPHT LAB - Clearing Deck (71)
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1
in dealcoholization, tissue section is immersed in __
ante-medium
2
raises the refractive index of the tissue
ante-medium
3
clearing agents must be miscible with both __
alcohol and paraffin wax
4
does not cause to much brittleness
benzene
5
best routine clearing agent
benzene
6
can cause aplastic anemia
benzene
7
used for large, thick, hard tissues
chloroform
8
slow acting
toxic to liver
does not make tissues translucent
tissues tend to float
chloroform
9
used for cleaning knives
xylene
10
diluent in mounting medium
xylene
11
most common clearing agent
xylene
12
for tissues less than 5 mm
xylene
13
useful for paraffin embedding
xylene
14
becomes milky/turbid if water is still present (incomplete dehydration)
xylene
15
overclearing causes tissue to become
hard and brittle
16
sub for xylene
toluene
17
subs for xylene/benzene
toluene
18
for CNS, skin, smooth muscle, cytological work
cedar oil/cedar wood oil
19
can leave tissue for a very long time w/o dehydration
cedar oil/cedar wood oil
20
penetrates well = best clearing agent
cedar oil/cedar wood oil
21
expensive and turbid
cedar oil/cedar wood oil
22
SLOW
Makes tissue brittle
can be faked
clove oil
23
prevents clove oil from becoming brittle
oil of bergamot
24
does not extract aniline dyes
oil of bergamot
25
can be used with celloidin prep
oil of bergamot
26
spanish hop oil or thyme oil or mix
oil of origanum
27
dissolves out aniline dyes
oil of origanum
28
does not dissolve celloidin
oil of origanum
29
other names of oil of wintergreen
methyl salicylate, artificial oil
30
