BACTE LEC - PSEUDOMONAS

Question 1

– water soluble blue green pigment

Answer 1

Pyocyanin

Question 2

– yellow fluorescing pigment

blue green pigment

Answer 2

Pyoverdin or fluorescein

Question 3

odor of pseudomonas aeruginosa

Answer 3

Grapelike or “corn tortilla-like odor

Question 4

odor of pseudomonas aeruginosa is due to

Answer 4

(production of 2-aminoacetophenone)

Question 5

Ability to grow at 42oC

Answer 5

pseudomonas aeruginosa

Question 6

Single polar flagellum

Answer 6

pseudomonas aeruginosa

Question 7

Grow very well on MAC & EMB

Answer 7

pseudomonas aeruginosa

Question 8

appearance of P. aeruginosa colonies

Answer 8

Large, rough, dull grayish, spreading

Question 9

oxidase test rxn of P. aeruginosa

Answer 9

Positive

Question 10

P. aeruginosa oxidize what sugar

Answer 10

glucose

Question 11

hemolytic patterns of P. aeruginosa

Answer 11

beta

Question 12

TSI rxn of P. aeruginosa

Answer 12

K/K

Question 13

vf of P. aeruginosa for attachment and conjugation

Answer 13

pili

Question 14

vf of P. aeruginosa for production of clot/foam

Answer 14

alginate

Question 15

appearance of P. aeruginosa on BA

Answer 15

green

Question 16

medium w/c enhances pigmentation of Pseudomonas

Answer 16

chrom agar

Question 17

diagnosis if Pseudomonas [aeruginosa] is an agent of severe wound infection in burn patients

Answer 17

uv light or woods light
wood’s lamp
[if it fluoresces,+]

Question 18

causes UTI
nosocomial pneumonia
septicemia
chronic lung infection
septic arthritis
destructive eye infections

Answer 18

P. aeruginosa

Question 19

causes swimmer’s ear

shanghai fever

Answer 19

P. aeruginosa

Question 20

pseudomonas also called

Answer 20

water bug

Question 21

capable of producing biofil which can be an agent of blindness

Answer 21

pseudomonas aeruginosa

Question 22

soil denitrifiers

Answer 22

p. stutzeri

Question 23

found in Soil
Marine water
Sewage
Stagnant water
Hospital instrumentation

Answer 23

p. stutzeri

Question 24

appearance of p. stutzeri on BA/MAC

Answer 24

dry wrinkled buff light brown

Question 25

tough and adherent

Answer 25

p. stutzeri

Question 26

starch hydrolysis reaction of p. stutzeri

Answer 26

+

Question 27

ADH reaction of p. stutzeri

Answer 27

-

Question 28

causes otitis media, conjunctivitis, septic arthritis

Answer 28

p. stutzeri

Question 29

A UTI agent proteolytic hydrolyzes gelatin

Answer 29

P. fluorescens

Question 30

causes infection of arms and legs | hospital environmental sources- sinks

Answer 30

P. putida

Question 31

all pseudomonas are motile except for

Answer 31

P. mallei

Question 32

``` Infects Horses Goats Sheep Donkeys ```

Answer 32

P. mallei

Question 33

Only nonmotile pseudomonad

Answer 33

P. mallei

Question 34

causes glanders disease

Answer 34

P. mallei

Question 35

also known as glanders bacilli

Answer 35

P. mallei

Question 36

appearance of colonies of p. mallei on BA

Answer 36

Colonies – smooth, cream to white on BA

Question 37

OXIDASE TEST reaction of p. mallei

Answer 37

Weakly oxidase +

Question 38

Fails to grow at 42C

Answer 38

P. mallei

Question 39

causes Melioidosis OR Vietnam time bomb

Answer 39

P. pseudomallei

Question 40

Cream to tan, wrinkled colonies

Answer 40

P. pseudomallei

Question 41

ADH +

Answer 41

P. pseudomallei