Flashcards in BACTE LEC - Misc Gram-negative Bacteria Deck (99):
1
Nonmotile
Pleomorphic coccobacilli or rods [2]
Pasteurella multocida
Haemophilus
2
require blood for growth
haemophilus
3
type of factor
provided by blood agar
hematin or hemin
Unknown
X factor
4
type of factor
provided by NAD or coenzyme 1
Vitamin
V factor
5
species of haemophilus w/c require only V factor
H. ducreyi*
H. parainfluenza
H. paraphrohaimolyticus
H. paraphrophilus
6
not adequate to isolate Haemophilus bc it releases NADase
Sheep blood agar
7
inactivates NAD, no NAD, no V factor
NADase (by sheep blood agar)
8
culture medium w/o NADase
horse or rabbit blood agar
9
type of agar
may release hematin and inactivate NADase
choc agar
10
method for the primary isolation of Haemophilus
staph streak
11
in staph streak method, growth near staph is __ [describe]
luxuriant
12
in staph streak, what organism provided nutrients for haemophiilus growth
staph
13
type of hemolysis by s. aureus that releases hematin
beta
14
phenomenon in which there are tiny colonies around staph colony
satellite phenomenon
15
pathogenic spp of Haemophilus
common isolate in CSF if there is meningitis esp among children
H. influenzae
16
VF of H. influenzae
Capsule
IgA protease
Adherence mechanism
Outer membrane components
17
vaccine for influenzae
Hib vaccine
18
clinical manifestations of H. influenzae [3]
meningitis
epiglottitis
bacterial tracheitis
19
Causative agent of acute contagious conjunctivitis
H. aegyptius
20
other name of acute contagious conjunctivitis
pinkeye/sore eye
21
also called Koch-Weeks Bacillus
H. aegyptius
22
general MoT of haemophilus spp except for
inhalation
23
MoT of ducreyi
sexual contact
24
CA of Brazilian purpuric fever (BPF)
H. influenzae biogroup aegyptius
25
causes chancroid or soft chancre
H. ducreyi
26
causes transmitted genital ulcer disease
H. ducreyi
27
causes suppurative, enlarged, draining, inguinal lymph nodes
H. ducreyi
28
sx for H. influenzae
blood, CSF, from upper and lower RT,
29
sx for ducreyi
vaginal swab
abscess drainage
30
sx for aegyptius
swab for conjunctivae
31
atmospheric requirements of haemophilus
5-10% CO2 - Capnophilic
32
microscopic appearance of ducreyi
shoal, school of fish
33
shoal of fish
loosely coiled clusters of organisms lined up in parallel or appearing as fingerprints
H. ducreyi
34
Beta hemolytic on SBA
Clumpy nonhomogenous appearance
ducreyi
35
MAC appearance: larger and more mucoid
ducreyi
36
CAP: translucent, tannish, moist, smooth convex
w/ mousy or bleachlike odor
ducreyi
37
odor of ducreyi on CAP
mousy or bleachlike
38
most misc gram (-) bacilli are __ [atmospheric requirement and location]
obligate intracellular
39
faintly staining coccobacilli
small grayish colonies on GCBA
Francisella
40
needs agglutination test and dfat for confirming its spp
Francisella
41
BSL number/level for francisella
3
42
Zoonotic [2]
>Brucella
>Francisella
43
Bioterrorism agent [2]
>Brucella
>Francisella
44
Serological Tests for Spp ID
[2]
>Brucella
>Francisella
45
CA of tularemia
potential agent of bioterrorism
F. tularensis
46
zoonotic infection: rabbits
F. tularensis
47
intracellular bacteria which resists phagocytosis
F. tularensis
48
pathology:
skin ulcers at infection site
lymph node infection [ulceroglandular]
lungs [pneumonic]
GI system [typhoidal]
F. tularensis
49
severe pathology by f. tularensis
pneumonic infection
50
causes diarrhea similar to Salmonellosis
F. tularensis
51
Causes rabbit fever
F. tularensis
52
diagnosis for F. tularensis
DFAT
agglutination testing
53
grows on BCYE and Thayer-Martin w/ 10% CO2
Slow grower [3-4 weeks]
Brucella
54
zoonotic: infects cows and cattles
Brucella
55
specimen for DX of brucella
BM, Blood
56
spp of brucella closely related with abortion among cows and cattles
B. abortus
57
diagnosis of brucella
cultures
58
spp of id of brucella
serological test= phage and dye sensitivty
59
requires phage and dye sensitivty
brucella
60
causative agent of whooping cough
B. pertussis
61
faintly staining gram(-) coccobacilli
francisella
bordetella
62
mercury droplet colonies
b. pertussis
63
culture media for b. pertussis
bordet gengou agar
regan-lowe [charcoal horse blod agar]
64
secondary stain is replaced with stronger dye since this organism is faintly staining
b. pertussis
65
Example of stronger dye
carbolfuchsin
66
3 stages of pathology of b. pertussis whooping cough
catarrhal
paroxysmal
convalescent
67
3 stages of pathology of b. pertussis whooping cough
very mild inflammation
small mucus secretions
catarrhal
68
3 stages of pathology of b. pertussis whooping cough
intermittent fever
body malaise
fullblown infection
paroxysmal
69
3 stages of pathology of b. pertussis whooping cough
recovery stage
convalescent
70
causes mild respiratory infections in human
weaker strain of bordetella
b. parapertussis
71
causes cough in dogs [bordetella]
b. bronchiseptica
72
grows well in nonselective agars but not MAC
P. multocida
73
very suscep to penicillin easy to treat
P. multocida
74
CA of cellulitis w/c may progress to osteomyelitis, joint infection, pneumonia
P. multocida
75
thin poorly stained Gram - bacilli
Legionella
76
requires L-cysteine for growth
Legionella
77
legionella requires ___ for growth
L-cysteine
78
CM for Legionella
BCYE
79
Does Legionella grow on SBA
No
80
CAP appearance of Legionella
tiny colonies
81
is Legionella sac or nonsac
assacharolytic
82
most biochem tests are negative
Legionella
83
not easily identified by routine tests
Legionella
84
1sr discovered in 1976, American Legion in Philadelphphia
Legionella
85
what causes Legionellosis, Legionnaire's Disease, Pontiac Fever
L. pneumophila serogroup 1
86
specimen for ___
secretion from lower RT
cough swab
bronchial swab
expectorated sputum
cough plate
L. pneumophila serogroup 1
87
unique Dx test for Legionella pneumophila
urine antigen test
88
other Dx test for Legionella pneumophila [3]
urine antigen test
DFAT
Nucleic acid probes
89
very small Gram - variable coccobacilli
Garnerella vaginalis
90
how to differentiate G. vaginalis from Lactobacillus
Lactobacillus is large Gram + bacilli
91
2 similar spp recovered in vaginal area or secretions
Lactobacillus
G. vaginalis
92
associated with bacterial vaginosis, pelvic inflammatory disease [PID], postpartum sepsis
G. vaginalis
93
presence of __ is suggestive of BV due to G. vaginalis
clue cells
94
squamous epithelial cells with intracellular G. vaginalis
clue cells
95
2 possible disease if there is lower abdominal pain
UTI
Vaginalis
96
Dx for G. vaginalis
Amsel and Nugent scoring system
97
____ are too sensitive, not ideal for ideal for diagnosing Bacterial vaginosis by G. vaginalis
cultures
98
used for reading the pap smear [for possible infections by G. vaginalis]
Amsel and Nugent scoring system
99