GPHT LAB - Apparatus II Flashcards Preview

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Flashcards in GPHT LAB - Apparatus II Deck (42)
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1

Shape of microtome knife
two sides: one flat and one concave

plane-concave

2

in plane concave knife, the one with lesser degree of concavity is used for __

celloidin section

3

in plane concave knife, the one with more degree of concavity is used for __

paraffin sections on sledge, rotary or rocking microtome

4

Shape of microtome knife
routinely used for frozen sections and extremely hard specimens embedded in paraffin wax

plane wedge

5

Shape of microtome knife
used for paraffin blocks being cut on the rotary microtome

plane wedge

6

length of plane wedge

100 mm

7

when not in use, where must the knife be stored

in its box

8

never lay the knife ___ on the bench

flat

9

never allow the knife to become badly " ___ "

nicked

10

it is advisable to have a 2nd knife for ___ and ___

cutting bone and hard tissue

11

the edge of the knife should be "___" daily before use

touch up

12

always use a ___, when required for sharpening

back

13

using a back for sharpening not necessary for what knives?

biconcave

14

cleaning material for the knife

xylene or toluene

15

never __ or __ a knife

lend or borrow

16

knife sharpener with a vibrating frosted glass plate

automatic knife sharpener

17

in the automatic knife sharpener, every __ strokes the blade is automatically turned over

4

18

automatic knife sharpener require the use of ___

abrasives

19

also called Cold Microtome

Cryostat

20

a cold chamber, which is kept at -20C

Cryostat

21

cold chamber of cryostat contains what microtome type

rotary

22

average temperature of the cold chamber/cryostat

-20C

23

used to cut frozen tissue

cryostat

24

replaced the carbon dioxide microtome for frozen sections

cryostat

25

thickness of tissue that can be cut by a cryostat

1-4 u

26

apparatus to prepare frozen tissue

cryostat

27

apparatus used to determine if tumor is benign or maignant

cryostat

28

RFS takes how many min

15 - 20

29

reagent in stations 1&2 of autotechnicon

10% formalin

30

an automatic tissue processor that performs fixation, dehydration clearing and paraffin infiltration

autotechnicon

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