BACTE LAB - Biochemical tests

Question 1

LIA Carbohydrate

Answer 1

glucose

Question 2

amino acid in LIA

Answer 2

Lysine

Question 3

ph indicator

Answer 3

bromcresol purple

Question 4

H2S indicator

Answer 4

ferric ammonium citrate

Question 5

Lysine decarboxylase +

Answer 5

K/K purple over purple

Question 6

Lysine decarboxylase -

Answer 6

K/A purple/yellow

Question 7

Deaminase -

Answer 7

R/A red/yellow

Question 8

H2S positive in LIA

Answer 8

blackening

Question 9

indol production test medium

Answer 9

tryptone broth

Question 10

indol production test negative result

Answer 10

yellow or brown ring

Question 11

indol production test reagent

Answer 11

Ehrlich’s or Kovac’s (paraaminobenzaldehyde)

Question 12

indol production test + result

Answer 12

red ring

Question 13

methyl red test medium

Answer 13

MRVP or Methyl Red Voges Proskauer medium

Question 14

methyl red test reagent

Answer 14

methyl red indicator

Question 15

methyl red test + result

Answer 15

red solution

Question 16

methyl red test - result

Answer 16

yellow solution

Question 17

voges proskauer test medium

Answer 17

MRVP medium

Question 18

voges proskauer test reagent

Answer 18

VP Reagent A (alpha naphthol in absolute methyl alcohol)

VP Reagent B (KOH w/ creatine)

Question 19

what is produced in the voges proskauer test

Answer 19

acetoin

Question 20

In the VP Test, acetoin is converted to?

Answer 20

diacetyl

Question 21

Citrate utilization test medium

Answer 21

simmon citrate agar

Question 22

Citrate utilization test indicator

Answer 22

bromthymol blue

Question 23

Citrate utilization test + result

Answer 23

prussian blue color

Question 24

Citrate utilization test - result

Answer 24

Green color

Question 25

urease test medium

Answer 25

urea broth

Question 26

urease test indicator

Answer 26

phenol red

Question 27

urease test positive result

Answer 27

pink to red

Question 28

urease test negative result

Answer 28

no color change

Question 29

SIM MEDIUM

Answer 29

Sulfide indol motility medium

Question 30

SIM Reagent

Answer 30

Ehrlich's or Kovac's

Question 31

Containers in water analysis

Answer 31

sterile glass bottles w/ round glass stoppers or screw caps

Question 32

Volume of water in water analysis

Answer 32

not less than 100 ml

Question 33

method in water analysis

Answer 33

multiple fermentation tube technique

Question 34

medium in water analysis

Answer 34

Brilliant Green Bile Lactose Broth

Question 35

tubes used in water analysis

Answer 35

durham loeffler hard glass

Question 36

double strength concentration

Answer 36

80 g/L

Question 37

single strength conc

Answer 37

40 g/L

Question 38

positive confirmed test result in water analysis

Answer 38

black colonies w/ metallic sheen on emb

Question 39

negative confirmed test result in water analysis

Answer 39

no black colonies w/ metallic sheen on emb

Question 40

positve completed test result

Answer 40

Gram (-) nonsporeforming bacilli presence of turbidity bubble/gas/space

Question 41

the results of the examinations of the replicate tubes and dilutions is expressed in terms of

Answer 41

most probable number (mpn)