SI LEC - Classes of Antibodies

Question 1

Most abundant immunoglobin,

Answer 1

IgG

Question 2

% IgG

Answer 2

75-80% of serum Ig

Question 3

S and MW of IgG

Answer 3

7S, MW=150,000

Question 4

Longest half life (23-25 days)

Answer 4

IgG

Question 5

Half life of IgG

Answer 5

23-25 days

Question 6

IgG which cross the placenta

Answer 6

IgG1, IgG3 and IgG4

Question 7

IgG least efficient in crossing the placenta

Answer 7

IgG2

Question 8

IgG most effective complement activator

Answer 8

IgG3:

Question 9

IgG High affinity for FcR on phagocytic cells, good for opsonization

Answer 9

IgG1 and IgG3

Question 10

FUNCTIONS
Provide immunity to the newborn
Fix complement
Coating antigen (opsonization)
Neutralizing toxins and viruses
Participate in agglutination and precipitation

Answer 10

IgG

Question 11

% IgM

Answer 11

5-10%

Question 12

S and MW of IgM

Answer 12

19S, MW= 970,000

Question 13

Shorter life span than IgG (about 10 days)

Answer 13

IgM

Question 14

Pentameric version is secreted, Monomeric on B cell surface

Answer 14

IgM

Question 15

IgM version in secretion

Answer 15

pentamer

Question 16

IgM version in B cell surface

Answer 16

Monomer

Question 17

5 monomeric units are held together by __ chains

Answer 17

J

Question 18

More efficient than IgG in complement activation

Answer 18

IgM

Question 19

Cannot cross the placenta

Answer 19

IgM

Question 20

Antigen-induced conformational changes in IgM

(2)

Answer 20

starfish or planar

staple or crab

Question 21

FUNCTIONS
Complement fixation
Opsonization
Agglutination
Toxin neutralization

Answer 21

IgM

Question 22

*First Ig of primary immune response

Answer 22

IgM

Question 23

IgG increased at what response (primary or secondary)

Answer 23

secondary

Question 24

conc of IgM in primary and secondary response

Answer 24

SAME

Question 25

Long lag phase Slow exponential increase in antibody Short lived response

Answer 25

Primary Immune Response

Question 26

Anamnestic response Short lag phase Antibody increase is more rapid Long lived response

Answer 26

Secondary Immune Response

Question 27

% IgA in serum IgG

Answer 27

10-15% of serum IgG

Question 28

S and MW of IgA

Answer 28

7S and MW=160 000

Question 29

Predominant Ig in secretions

Answer 29

IgA

Question 30

``` IgA Subclass found in serum, mainly monomeric, polymers possible not common though ```

Answer 30

IgA1

Question 31

``` IgA Subclass found in secretions, as dimer, w/ J-chain, w/ secretory component ```

Answer 31

IgA2

Question 32

IgA1 and IgA2: differ in __ aa (13 of which is located in the hinge region)

Answer 32

22

Question 33

__ more resistant to bacterial proteinases than IgG1

Answer 33

IgA2

Question 34

MW of IgA secretory component

Answer 34

70 000

Question 35

IgA Structure | attached to Fc region around the hinge of the alpha chain,

Answer 35

Secretory component

Question 36

IgA Structure | facilitates transport of IgA to mucosal surface,

Answer 36

Secretory component

Question 37

IgA Structure | masks sites that would be susceptible to protease cleavage

Answer 37

Secretory component

Question 38

not capable of C’ activation

Answer 38

IgA

Question 39

FUNCTIONS of Ig Patrol mucosal surfaces Neutralize toxins Helps prevent bacterial adherence to mucosa

Answer 39

IgA

Question 40

extremely scarced in the serum (0.001%)

Answer 40

IgD

Question 41

% IgD in serum

Answer 41

0.001%

Question 42

half life of IgD

Answer 42

2-3 days

Question 43

MW of IgD

Answer 43

MW=180,000

Question 44

Expressed on B-cell Surface - Play a role in B cell activation - Regulate B cell maturation and differentiation

Answer 44

IgD

Question 45

Least abundant (0.0005%)

Answer 45

IgE

Question 46

% IgE

Answer 46

0.0005%

Question 47

S and MW of IgE

Answer 47

8S, MW=190,000

Question 48

Heat labile

Answer 48

IgE

Question 49

Nuisance ab

Answer 49

IgE

Question 50

Not capable of C’ fixation, agglutination, opsonization, or crossing the placenta

Answer 50

IgE

Question 51

Binds mast cells and basophils thru Fc(EPSILON)R (CH3)

Answer 51

IgE

Question 52

Binding causes degranulation (Histamine release)

Answer 52

IgE

Question 53

IgE Binds mast cells and basophils thru __

Answer 53

Fc(EPSILON)R (CH3)

Question 54

H chain genes are located on chromosome __

Answer 54

14

Question 55

kappa chain genes are on chromosome _

Answer 55

2

Question 56

the lambda chain genes are on chromosome __

Answer 56

22

Question 57

IgG with extra constant chain

Answer 57

IgG and IgM

Question 58

extra constant chain of IgG

Answer 58

CH2

Question 59

extra constant chain of IgM

Answer 59

CH3

Question 60

extra constant chain of IgG are sites for __

Answer 60

complement binding

Question 61

Ig implicated in HDN

Answer 61

IgG

Question 62

agglutination, nature of Ag

Answer 62

INSOLUBLE

Question 63

precipitation, nature of Ag

Answer 63

SOLUBLE

Question 64

conformation of IgM when unbound to Ab

Answer 64

Starfish

Question 65

conformation of IgM when bound to Ab

Answer 65

Crab

Question 66

patrol Ab

Answer 66

IgA

Question 67

protects IgA from enzymes that may degrade it

Answer 67

secretory component

Question 68

with polyIg receptor

Answer 68

IgA

Question 69

Ab diversity | different chromosomes control different parts of Ab

Answer 69

Tonegawa

Question 70

theory 1.) the lock and key concept of the fit of ab for ag 2.) the idea that an ag selected cell w/ built-in capacity to respond to it.

Answer 70

Ehrlich’s Side Chain Theory

Question 71

-individual lymphocytes are genetically pre-programmed to produce one type of Ig and that a specific antigen finds or selects those particular cells capable of responding to it, causing them to proliferate

Answer 71

Clonal Selection Theory

Question 72

3 Gene groups of HC genes

Answer 72

VH, D, J

Question 73

How many VH

Answer 73

39

Question 74

How many D

Answer 74

23

Question 75

How many J

Answer 75

6