Flashcards in SI LEC - Classes of Antibodies Deck (75):
1
Most abundant immunoglobin,
IgG
2
% IgG
75-80% of serum Ig
3
S and MW of IgG
7S, MW=150,000
4
Longest half life (23-25 days)
IgG
5
Half life of IgG
23-25 days
6
IgG which cross the placenta
IgG1, IgG3 and IgG4
7
IgG least efficient in crossing the placenta
IgG2
8
IgG most effective complement activator
IgG3:
9
IgG High affinity for FcR on phagocytic cells, good for opsonization
IgG1 and IgG3
10
FUNCTIONS
Provide immunity to the newborn
Fix complement
Coating antigen (opsonization)
Neutralizing toxins and viruses
Participate in agglutination and precipitation
IgG
11
% IgM
5-10%
12
S and MW of IgM
19S, MW= 970,000
13
Shorter life span than IgG (about 10 days)
IgM
14
Pentameric version is secreted, Monomeric on B cell surface
IgM
15
IgM version in secretion
pentamer
16
IgM version in B cell surface
Monomer
17
5 monomeric units are held together by __ chains
J
18
More efficient than IgG in complement activation
IgM
19
Cannot cross the placenta
IgM
20
Antigen-induced conformational changes in IgM
(2)
starfish or planar
staple or crab
21
FUNCTIONS
Complement fixation
Opsonization
Agglutination
Toxin neutralization
IgM
22
*First Ig of primary immune response
IgM
23
IgG increased at what response (primary or secondary)
secondary
24
conc of IgM in primary and secondary response
SAME
25
Long lag phase
Slow exponential increase in antibody
Short lived response
Primary Immune Response
26
Anamnestic response
Short lag phase
Antibody increase is more rapid
Long lived response
Secondary Immune Response
27
% IgA in serum IgG
10-15% of serum IgG
28
S and MW of IgA
7S and MW=160 000
29
Predominant Ig in secretions
IgA
30
IgA Subclass
found in serum, mainly monomeric, polymers possible not common though
IgA1
31
IgA Subclass
found in secretions, as dimer, w/ J-chain, w/ secretory component
IgA2
32
IgA1 and IgA2: differ in __ aa (13 of which is located in the hinge region)
22
33
__ more resistant to bacterial proteinases than IgG1
IgA2
34
MW of IgA secretory component
70 000
35
IgA Structure
attached to Fc region around the hinge of the alpha chain,
Secretory component
36
IgA Structure
facilitates transport of IgA to mucosal surface,
Secretory component
37
IgA Structure
masks sites that would be susceptible to protease cleavage
Secretory component
38
not capable of C’ activation
IgA
39
FUNCTIONS of Ig
Patrol mucosal surfaces
Neutralize toxins
Helps prevent bacterial adherence to mucosa
IgA
40
extremely scarced in the serum (0.001%)
IgD
41
% IgD in serum
0.001%
42
half life of IgD
2-3 days
43
MW of IgD
MW=180,000
44
Expressed on B-cell Surface
-Play a role in B cell activation
-Regulate B cell maturation and differentiation
IgD
45
Least abundant (0.0005%)
IgE
46
% IgE
0.0005%
47
S and MW of IgE
8S, MW=190,000
48
Heat labile
IgE
49
Nuisance ab
IgE
50
Not capable of C’ fixation, agglutination, opsonization, or crossing the placenta
IgE
51
Binds mast cells and basophils thru Fc(EPSILON)R (CH3)
IgE
52
Binding causes degranulation (Histamine release)
IgE
53
IgE Binds mast cells and basophils thru __
Fc(EPSILON)R (CH3)
54
H chain genes are located on chromosome __
14
55
kappa chain genes are on chromosome _
2
56
the lambda chain genes are on chromosome __
22
57
IgG with extra constant chain
IgG and IgM
58
extra constant chain of IgG
CH2
59
extra constant chain of IgM
CH3
60
extra constant chain of IgG are sites for __
complement binding
61
Ig implicated in HDN
IgG
62
agglutination, nature of Ag
INSOLUBLE
63
precipitation, nature of Ag
SOLUBLE
64
conformation of IgM when unbound to Ab
Starfish
65
conformation of IgM when bound to Ab
Crab
66
patrol Ab
IgA
67
protects IgA from enzymes that may degrade it
secretory component
68
with polyIg receptor
IgA
69
Ab diversity
different chromosomes control different parts of Ab
Tonegawa
70
theory
1.) the lock and key concept of the fit of ab for ag 2.) the idea that an ag selected cell w/ built-in capacity to respond to it.
Ehrlich’s Side Chain Theory
71
-individual lymphocytes are genetically pre-programmed to produce one type of Ig and that a specific antigen finds or selects those particular cells capable of responding to it, causing them to proliferate
Clonal Selection Theory
72
3 Gene groups of HC genes
VH, D, J
73
How many VH
39
74
How many D
23
75