Flashcards in CC LAB - TPAG Deck (18)
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1
Based on measurement of nitrogen content
Kjeldahl Method
2
Reference method
Kjeldahl Method
3
Digesting agent of Kjeldahl Method
H2SO4
4
Most commonly used
Biuret Method
5
Need two peptide bonds and an alkaline medium
Biuret Method
6
Composition of Biuret Reagent:
a) Copper sulfate- provides the cupric ion that complexes with the peptide bonds
b) Potassium sodium tartrate (Rochelle’s salt)- prevents precipitation in alk solution
c) Potassium iodide- prevents autoreduction of copper
7
- important in maintaining plasma oncotic pressure
Albumin
8
family of globular proteins that have higher molecular weights than albumin and are insoluble in pure water
Globulin-
9
Inverted A:G ratio means
presence of myeloma
10
Immediate response occurs with stress or inflammation caused by infection, injury or surgical trauma
Acute inflammatory response
11
• normal or ↓ albumin
• ↑ α1 and α2 globulins
Acute inflammatory response
12
Late response is correlated with chronic infection
(autoimmune diseases, chronic liver disease, chronic infection, cancer)
Chronic inflammatory response
13
• normal or ↓ albumin
•↑α1 or α2 globulins
•↑↑ γ globulins
Chronic inflammatory response
14
Cirrhosis can be caused by [2]
chronic alcohol abuse or viral hepatitis
15
↓ albumin
• ↓ α1, α2 and β globulins
• ↑ Ig A in γ-fraction
Liver damage - Cirrhosis
16
the kidney damage illustrates the long term loss of lower molecular weight proteins
Nephrotic syndrome
17
retention of higher molecular weight proteins (↑↑ α2-macroglobulin and ↑β-globulin)
Nephrotic syndrome
18
