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Flashcards in basic histologic techniques Deck (67)
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1

12 Steps of Histologic Techniques

numbering
receiving of specimens
fixation
dehydration
clearing
infiltration
embedding
blocking and trimming
sectioning
staining
mounting
labelling

2

first and most important step
logging in a log book the details of the tissue

numbering

3

tissue specimens should be properly labeled and with a corresponding request
tissue s will be described by the pathologist (gross description)
tissues will be cut by the pathologist

receiving of specimens

4

tissues are immersed in a fixative to prevent decomposition and preserve structure

fixation

5

removal of excess water using ascending grades of alcohol

dehydration

6

removal of excess alcohol
makes tissues clear/transparent
prepares tissues for paraffin impregnation

clearing/dealcoholization

7

tissue cavities are saturated w/ paraffin wax

infiltration

8

impregnated tissue is oriented and embedded in paraffin block to form a tissue block

embedding

9

separation of embedded tissues into blocks
removal of excess paraffin wax using knives

blocking and trimming

10

very thin sections of tissue are cut using microtome

sectioning

11

using H& E (routine stain)

staining

12

stained tissue sections on a slide are added w/ mounting medium and covered w/ coverslip

mounting

13

final step in tissue preparation

labeling

13

Responsible for transcribing the dictations of the pathologist

Medical technologist

14

Used in labeling slecimens

Pencil

15

Provides the gross description
Cuts of sections the specimen when received

Pathologist

16

Technique done to kill microorganism in the specimen

Fixation

17

Routinely used fixative

Formalin (10%) or formaldehyde

18

Stock solution of formalin that must be diluted

37% formalin

19

Agent used in dehydration

Increasing grades of Ethanol or ethyl alcohol

20

Effect of clearing to tissues

Tissues become translucent or transparent

21

Most common clearing agent

Xylol or xylene

22

Melting point of paraffin wax

56-57C

23

Ways of performing infiltration

Manual
Using automatic tissue processor

24

Another name for infiltration

Impregnation

25

Performs fixation to infiltration (steps 2-5)
Has a basket wherein tissue cassette will be placed

Automatic tissue processor

26

How long do cutting to infiltration take?

1 day

27

The act of placing impregnated tissue in the embedding mold

Orientation

28

Examples of embedding mold

Paper boat
Plastic mold
Ice cube tray

29

Separation of embedded tissues into blocks
Separating small block from big block

Blocking

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