Bacte Lec - Staph Flashcards
1
Q
gram reaction of staph
A
positive
2
Q
color of staph in gram stain
A
positive
3
Q
gram + spherical cells which normally colonize the skin
A
micrococcus
4
Q
morphology of micrococcus
A
cubes of 8
5
Q
coagulase reaction of micrococcus
A
negative
6
Q
coccus bacteria in marine environment
rarely assoc with human infection
A
planococcus
7
Q
test to diff strep and staph
A
catalase test
8
Q
catalase rxn of stapg
A
+
9
Q
all staph are [aerobe or anaerobe]
A
facultative anaerobe
10
Q
only obligate anaerobe staph
A
S. saprophyticus
11
Q
hemolytic rxn of staph
A
beta or non-hemolytic
12
Q
reaction of staph in catalase [describe]
A
bubble formation
13
Q
catalase test not used on blood agar bec
A
blood has coagulase
14
Q
rxn of staph on SIM
A
confined; not turbid
15
Q
common isolate on wound infection
A
suppurative type of infection [pus-forming]
16
Q
habitant of skin and mucous membrane
A
staph
17
Q
found in environment on dust
inanimate objects
A
staph
18
Q
single most impt test to differentiate pathogenic staph from other species
A
coagulase test
19
Q
coagulase producing staph
A
Hyicus Aureus Delphini Intermedius Schleiferi
20
Q
all coagulase producing staph are animal species except
A
aureus
21
Q
virulence factors of staph [5]
A
enterotoxin exfoliative toxin cytolytic toxin enzymes protein A
22
Q
type of VF
heat stable
A
enterotoxin
23
Q
type of enterotoxin that are resistant to gastric acid and digestive juices
A
enterotoxin A and enterotoxin D
24
Q
produce acid in the form of HCl
A
parietal cells
25
type of VF
phage group II
involvement of skin
formation of blisters
Exfoliative toxin
26
type of exfoliative toxin Observed among tampon
Toxic shock syndrome toxin 1
27
type of exfoliative toxin
sloughing off of epidermal layer of skin
scalded skin syndrome [Ritter disease]
epidermolytic
28
staph infection most observed where
skin
29
type of cytolytic toxin
| platelets destroyed
alpha toxin/ hemolysin
30
type of cytolytic toxin
injury on most cells on culture and to leukocytes
less lethal
delta hemolysin
31
another name for leukocidin
Panton-Valentine
32
type of cytolytic
lethal to PMN [macrophage]
invasiveness of organism
Leukocidin
33
another name for beta hemolysin
hot cold lysin
34
attacks sphingomyelin of RBC membrane
beta hemolysin
35
enzymes as VF [3]
Coagulase
Hyaluronidase
Lipase
36
type of enzyme
clumping factor
virulence marker
coagulase
37
type of enzyme
coagulase + and coagulase -
acts on fats and oils
responsible for colonization
lipases
38
type of enzyme
hydrolyze hyaluronic acid
with exfoliation
tries to make skin loose so bacteria can spread
hyaluronidase
39
another name for hyaluronidase
Durans-Reynal Factor or Spreading factor
40
type of VF with phagocytic activity
protein A
41
epidemiology of staph [3]
anterior nares of human carriers
hospital outbreaks [common nosocomial infections]
infant nurseries, burn units, invasive procedures
42
transmission of staph [2]
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direct contact
community-acquired infections
poor hygiene
fomite transmission [nonliving]
carriers
```
43
how to prevent s.aureus infection
hand washing
44
Cutaneous infection [4]
bullous impetigo
boils
furuncles
carbuncles
45
toxin induced diseases [4]
food poisoning [refrigerated foods rich with creamy sauces & mayonnaise]
toxic shock syndrome
scalded skin syndrome
toxic epidermal necrolysis
46
meaning of SSS
Scalded Skin Syndrome
47
meaning of TEN
toxic epidermal necrolysis
48
resolution for toxic induced diseases
steroid therapy
49
disease observed among young menstruating females
toxic shock syndrome
50
common among sexually active young women
S. saprophyticus
51
also seen in older men with indwelling catheter
S. saprophyticus
52
resistant to novobiocin
S. saprophyticus
53
most common hospital acquired UTI
catheter infection
54
causes UTI
S. saprophyticus
55
associated with pyelonephritis and cystitis
S. saprophyticus
56
a multisystemic disease characterized by high fever, hypotension, shock
other conditions are pneumonia, endocarditis, osteomyelitis, septic arthritis
toxic shock syndrome
57
isolation and identification culture media used [4]
thioglycollate broth
MSA
PEA
CNA
58
CNA meaning
columbia colistin nalidixic agar
59
PEA meaning
phenylethyl alcohol agar
60
MSA meaning
mannitol salt agar
61
cm which differentiates S. aureus from other staph
MSA
62
cm which eliminates Gram (-) only gram + is allowed to grow
PEA
63
colony size of aureus, epidermidis, saprophyticus
pinhead
64
type of staph beta hemolytic
aureus
65
type of staph non-hemolytic [2]
epidermidis and saprophyticus
66
color of colonies of aureus
yellow, tan, orange
67
color of colonies of epidermidis
white
68
color of colonies of saprophyticus
yellow
69
a clear zone indicates
hemolytic rxn
70
substrate of catalase test
3% hydrogen peroxide
71
genus + for catalase
staph
72
genus - for catalase
strep
| enterococcus
73
blood used in coagulase test
citrated blood
74
coagulase + bacteria
s. aureus
75
coagulase - bacteria
epidermidis or saprophyticus
76
novobiocin susceptible
epidermidis
77
novobiocin resistant
saprophyticus
78
coagulase test - for cell bound coagulase
slide test
79
rgts in slide test
human, rabbit, dog plasma
80
type of glass slide used in slide test
smoked glass slide
81
coagulase test for free coagulase
tube coagulase test
82
number of hours of incubation for tube coag test
4 hrs
83
why is there a need to check every time for the clot during the whole incubation period
S. aureus has hyaluronidase which can liquefy the clot = false negative
84
in msa, what does a yellow color mean
acid fermentation/LF
85
in msa, what does a red color mean
alkaline
86
what type of test rxn involves clearing of toluidine blue
DNAse Test Reaction
87
base medium of DNAse Test Reaction
nutrient agar
88
reagent in DNAse Test Reaction
dilute HCl
89
in DNAse Test Reaction, it is an indication of nucleic acid hydrolysis
blue green to pink
90
no clearing means what in DNAse Test Reaction
DNA not utilized
91
(+) in DNAse Test Reaction means
Hydrolysis of DNA
92
agar in gelatinase test
gelatin
93
reaction of gelatinase producing organism in gelatinase test
gelatin will liquefy
94
test to differentiate saprophyticus from other species
thermostable nuclease test/ novobiocin testing
95
s. aureus enzyme stable at what temp
40C
96
Other methods: rapid testing
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SEROSTAT
Slide Test
Staphylatex
Accu-staph
staphurex
staphyloslide
hemastaph
staphase
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