MYCOVIRO - CH 59 Book Pt 2 Flashcards Preview

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Flashcards in MYCOVIRO - CH 59 Book Pt 2 Deck (64)
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1

Vaginal Samples
-transported to lab within ? hrs using culture transport swabs

24

2

Vaginal Samples
-transported to lab within 24 hrs using __

culture transport swabs

3

vaginal samples: both __ and __ agars be plated

selective and inhibitory

4

vaginal samples must be screened for __, incubated at 30C for 7 days

yeasts

5

vaginal samples must be incubated at (temp and how many days)

30C for 7 days

6

what urine sx is unacceptable for culture

24-hr urine

7

urine sample media must contain what ?

antibacterial agents

8

All tissues must be processed before culturing by: (2)

• Mincing
• Or placement in high speed lab blender

9

express the cytoplasmic contents of cells using pressure from the action of rapidly moving metal paddles

stomacher lab blender

10

what specimen may be placed directly onto the surface of appropriate culture media

BM

11

For optimal recovery, a battery of media should be used:
(2)

• Media with and without cycloheximide
• Media with and without antibacterial agent (not necessary if specimen is from a sterile site)

12

Agar plates – preferred because (3)

• Provide better aeration
• Larger surface area for better isolation of colonies
• Greater ease of handling

13

agar tends to dehydrate during extended incubation period
--minimized using: ??

culture dishes containing at least 40 mL agar and placing them in a humidified incubator

14

ADV OF SCREW-CAPPED CULTURE TUBES

• Easily stored
• Require less space for incubation
• Easily handled
• Lower dehydration rate
• Less hazardous

15

DISADV OF SCREW-CAPPED CULTURE TUBES

• Poor colony isolation
• Reduced SA
• Tendency to promote anaerobiosis

16

if using screwcapped culture tubes, after inoculation, must be placed in a horizontal position for at least ??? hours to allow the specimen to absorb the agar surface and avoid settling at the bottom of tube

1-2

17

?? tubes unsatisfactory for fungal cultures

cotton-plugged tubes

18

cultures should be examined at least how many times weekly during incubation

3x

19

– added to any media that contain or lack antibiotics
-if it is included, a medium w/o it must also be used
-partially or completely inhibits…

Cycloheximide

20

– recommended method for direct microscopic examination

KOH preparation

21

- better than KOH preparation
-slides are observed using 1) fluorescent microscopy (optimal because fungal cells fluoresce) or 2) bright field microscopy

Calcoflour white stain

22


Serology testing - may be useful for (4, CHAB)

• Cryptococcus
• Blastomyces
• Histoplasma
• Aspergillus

23

– useful but not for immunocompromised patients (incapable of producing a measurable humoral response)


Antibody testing

24

– sensitive method, difficult to perform and interpret
-requires a delay in testing that extends from exposure to onset of symptoms
-detection of antibody can take 2 to 3 months
-cross reactions with other fungal antibodies may be a problem

Complement fixation (CF)

25

– simple, cost-effective procedure
-100% specific, relatively insensitive, not used as a screening tool
-requires 2-3 weeks to be positive

Immunodiffusion testing

26

– frequently negative in immunocompromised patients especially early in the infection


Enzyme immunoassays

27

- not accepted as a routine diagnostic tool in mycology

Molecular Detection

28

– biophysical method that reduces time required to specifically identify fungal organisms

MALDI-TOF

29

MALDI-TOF MEANING

Matrix-Assisted Laser Desorption Ionization

30

For Yeast ID
Many automated and semiautomated commercial systems have been designed with WHAT tests because C and N source differences are key to yeast differentiation)

assimilation and fermentation

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