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Flashcards in BACTE LAB - AFS Deck (61)
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1

Slides must be

new
clean
free from grease
free from fingerprints
unscratched

2

what to write on the frosted end of the slide

laboratory register serial number
order number of the sputum specimen

3

type of pencil to use on unfrosted slides

diamond pencil, left end of the slide

4

what part of the sputum must be taken

yellowish purulent part

5

smear shape

oval

6

smear size

2 cm wide x 3 cm length

7

the smear size corresponds to how many visual fields

150, to be counted in one length

8

if wire loop was used instead of applicator stick, dip it in ___

alcohol washed-sand bottle

9

clean the wire loop in a flask containing ___ before sterilizing in a flame

70% - 90% alcohol with glass beads or sand

10

for accidental contamination with sputum,
hand:

wash with soap and water

11

for accidental contamination with sputum,
cloth:

spray with 5% phenol OR
Soak in disinfectant OR
Autoclave OR
Boil

12

for accidental contamination with sputum,
Surface of container,

wear rubber gloves before handling it

13

for accidental contamination with sputum,
bench of floor

spray with 5% phenol or Lysol

14

How to dry the smears

air dry, not in direct sunlight or over a flame

15

Elements of Quality Smear

Quality of sputum
size of smear
thickness of smear
evenness of smear
staining technique
cleanness of slide

16

What will happen if during fixation the slide was heated for too long or kept stationary

smear will be scorched

17

a good stained smear by ZN method shows ___

strong red AFB, against weak blue background

18

It is not possible to decolorize if _____ are used

cold watery acids

19

To prepare 0.3% Ziehl's carbol fuchsin working solution, mix

10 ml solution A and 90 ml solution B

20

Solution A of Ziehl's carbol fuchsin

Fuchsin Alcoholic Stock Solution

21

Solution A of Ziehl's carbol fuchsin components

3g basic fuchsin
95% alcohol UP to 100 mL

22

Solution A of Ziehl's carbol fuchsin NAME

Aqueous Phenol Solution

23

Solution A of Ziehl's carbol fuchsin components

Phenol crystals 5g
Distilled water up to 90 mL

24

Before adding water, liquefy the phenol crystals by

gentle heating

25

Decolorizing Agent Solutions

Acid-alcohol solution
25% Aqueous Sulfuric Acid Solution

26

Acid-alcohol solution components

95% alcohol --- 970 mL
35% HCl --- 30 mL

27

25% Aqueous Sulfuric Acid Solution components

distilled water 300 ml
conc. sulfuric acid 100 ml

28

0.3% Methylene Blue Counterstaining Solution components

0.3 g methylene blue chloride
distilled water up to 100 mL

29

ZN method requires staining for ___ min

10

30

ZN method requires decolorizing for ___ min

approx 3 min

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