Flashcards in Para Lab - Quiz 1 Deck (49)
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1
– most common specimen
stool sx
2
amount of stool collected (Formed)
2-5 g
about size of walnut or thumb
3
must be labelled with
Name
Age
Date and time of collection
4
how many stool specimens are collected in different days to increase chances of detecting the parasite
3
5
stool sx must be accompanied with
a lab request
6
brown color of stool due to
stercobilin
7
• Gross abnormalities – presence of abnormal structures such as
Mucus
o Pus
o Proglottids (Segment of a tapeworm)
o Blood
8
uses iodine and saline wet preparations for screening purposes
DFS
9
for detection of helminths (parasitic worms) & protozoans
DFS
10
For detection of trophozoite and cyst stages
DFS
11
Disadv of DFS
not sensitive because of very small amount of Sx used
12
can hide fecal organisms
Fecal debris
13
ADV of conc technique
-advantage: increases the chances of parasite detection because a larger amount of stool is used
14
DISADV of conc technique
-disadvantage: cannot preserve or detect trophozoites because they are easily destroyed
15
2 methods of conc technique
flotation
sedimentation
16
used for small eggs or cysts that tend to float
flotation
17
-principle of flotation
all eggs and cysts will float
18
flotation not suitable for protozoans but useful for demonstration of helminthic eggs
brine flotation
19
-Sp Gr of brine solution
= 1.20
20
measures specific gravity
Refractometer
21
Advantages of brine
Inexpensive
• Applicable in field work
22
Disadvantages of brine
Badly shrunken morphology of some eggs and cysts
Eggs of Clonorchis and Opistorchis do not float
23
useful for: recovery of protozoan cysts and helminth eggs
ZnSO4 flotation
24
sp gr in zinc sulfate flotation
Sp Gr = 1.18 – 1.20
25
ZnSO4 flotation NOT useful for
• Large trematode eggs
• Some tapeworm (cestode) eggs
• Ascaris lumbricoides unfertilized eggs
26
useful for detection of Cryptosporidium
Sheather’s Sugar Flotation
27
Solution added in Sheather’s Sugar Flotation
sucrose-phenol solution
28
used for heavy or large eggs or cysts
sediments
29
FECT meaning
Formalin Ether Concentration Technique
30
