Bacte Lec - Stains

Question 1

Capsule color

Hiss stain

Answer 1

light violet

Question 2

Capsule color

Tyler stain

Answer 2

light violet

Question 3

Capsule color

Muir stain or Welchs

Answer 3

light violet

Question 4

capsule and background color

India ink or nigrosin

Answer 4

capsule: Unstained
bg: black

Question 5

capsule and background color

Congo red

Answer 5

capsule: Unstained
bg: blue

Question 6

capsule and background color

Anthony’s stain

Answer 6

capsule: Unstained
bg: purple

Question 7

granule color

Albert stain

Answer 7

Blue black

Question 8

Neisser stain granule color

Answer 8

Dark blue

Question 9

Lindegren’s stain granule color

Answer 9

Reddish brown

Question 10

Loeffler’s alkaline methylene blue (LAMB) granule color

Answer 10

dark blue

Question 11

Wayson’s stain

Answer 11

bipolar bodies (appear to be spore) are red

Question 12

Stain for flagella (4)

Answer 12

• Leifson stain
• Gray’s method
• Silver technique
• Caesares Gil

Question 13

endospore color

Fulton Schaeffer’s method

Answer 13

green

Question 14

endospore color

Dorner’s stain

Answer 14

red

Question 15

endospore color

Wirtz Conklin

Answer 15

green

Question 16

Stain for Rickettsia (3)

Answer 16

• Castaneda – blue
• Machiavelo – red
• Giemsa – blue

Question 17

Stain for Chlamydia (3)

Answer 17

• Gimenez – inclusion bodies are red
• Machiavello
• Giemsa

Question 18

Stain for spirochetes

Fontana Tribondeau

Answer 18

dark brown or black spirochetes

Question 19

Levaditi Silver Impregnation

Answer 19

– black

Question 20

India Ink negative stain

Answer 20

– unstained spirochetes, black background

Question 21

Stain for mycoplasma

• Dienes stain –

Answer 21

blue

Question 22

Stain for cell wall

• Victoria blue dye –

Answer 22

blue

Question 23

Stain for DNA

• Feulgen stain –

Answer 23

greenish or with green tinge

Question 24

chemical used in Kinyoun’s method

Answer 24

tergitol

Question 25

function of tergitol

Answer 25

reduces surface tension, promotes penetration or attachment

Question 26

alters decolorizer by weakening it to differentiate these 2 species: M. tuberculosis and M. smegma

Answer 26

Pappenheim’s method

Question 27

method that differentiates M. tuberculosis and M. leprae

Answer 27

Baumgarten's method

Question 28

stains M. leprae in tissue sections

Answer 28

Wadefite Technique

Question 29

staining technique for bacteria in tissue sections

Answer 29

Brown and Brenn technique

Question 30

modified Grams stain

Answer 30

Brown and Brenn technique

Question 31

stains for flagella (4)

Answer 31

Leifson Stain Caesares Gil Silver technique Gray's method

Question 32

Stains for acid-fast bacilli

Answer 32

``` Ziehl-Neelsen Kinyoun's Truant's fluorescence technique Flickerson and Kammer's fluorescence technique Pappenheim method Baumgarten's method Wadefite technique ```

Question 33

acid-fast bacilli stain that differentiates between M. leprae and M. tuberculosis

Answer 33

Baumgarten's method

Question 34

Baumgarten's method differentiates these two species

Answer 34

M. leprae and M. tuberculosis

Question 35

Pappenheim's method differentiates these 2 species

Answer 35

M. tuberculosis and M. smegma

Question 36

color of M. tuberculosis in Pappenheim's method

Answer 36

red

Question 37

color of M. smegma in Pappenheim's method

Answer 37

blue

Question 38

color of M. leprae in Baumgarten's method

Answer 38

red

Question 39

color of M. tuberculosis in Baumgarten's method

Answer 39

blue