Flashcards in Bacte Lec - Stains Deck (39):
1
Capsule color
Hiss stain
light violet
2
Capsule color
Tyler stain
light violet
3
Capsule color
Muir stain or Welchs
light violet
4
capsule and background color
India ink or nigrosin
capsule: Unstained
bg: black
5
capsule and background color
Congo red
capsule: Unstained
bg: blue
6
capsule and background color
Anthony’s stain
capsule: Unstained
bg: purple
7
granule color
Albert stain
Blue black
8
Neisser stain granule color
Dark blue
9
Lindegren’s stain granule color
Reddish brown
10
Loeffler’s alkaline methylene blue (LAMB) granule color
dark blue
11
Wayson’s stain
bipolar bodies (appear to be spore) are red
12
Stain for flagella (4)
• Leifson stain
• Gray’s method
• Silver technique
• Caesares Gil
13
endospore color
Fulton Schaeffer’s method
green
14
endospore color
Dorner’s stain
red
15
endospore color
Wirtz Conklin
green
16
Stain for Rickettsia (3)
• Castaneda – blue
• Machiavelo – red
• Giemsa – blue
17
Stain for Chlamydia (3)
• Gimenez – inclusion bodies are red
• Machiavello
• Giemsa
18
Stain for spirochetes
Fontana Tribondeau
dark brown or black spirochetes
19
Levaditi Silver Impregnation
– black
20
India Ink negative stain
– unstained spirochetes, black background
21
Stain for mycoplasma
• Dienes stain –
blue
22
Stain for cell wall
• Victoria blue dye –
blue
23
Stain for DNA
• Feulgen stain –
greenish or with green tinge
24
chemical used in Kinyoun's method
tergitol
25
function of tergitol
reduces surface tension, promotes penetration or attachment
26
alters decolorizer by weakening it to differentiate these 2 species: M. tuberculosis and M. smegma
Pappenheim’s method
27
method that differentiates M. tuberculosis and M. leprae
Baumgarten's method
28
stains M. leprae in tissue sections
Wadefite Technique
29
staining technique for bacteria in tissue sections
Brown and Brenn technique
30
modified Grams stain
Brown and Brenn technique
31
stains for flagella (4)
Leifson Stain
Caesares Gil
Silver technique
Gray's method
32
Stains for acid-fast bacilli
Ziehl-Neelsen
Kinyoun's
Truant's fluorescence technique
Flickerson and Kammer's fluorescence technique
Pappenheim method
Baumgarten's method
Wadefite technique
33
acid-fast bacilli stain that differentiates between M. leprae and M. tuberculosis
Baumgarten's method
34
Baumgarten's method differentiates these two species
M. leprae and M. tuberculosis
35
Pappenheim's method differentiates these 2 species
M. tuberculosis and M. smegma
36
color of M. tuberculosis in Pappenheim's method
red
37
color of M. smegma in Pappenheim's method
blue
38
color of M. leprae in Baumgarten's method
red
39