Bacte Lec - Stains Flashcards Preview

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Flashcards in Bacte Lec - Stains Deck (39)
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1

Capsule color
Hiss stain

light violet

2

Capsule color
Tyler stain

light violet

3

Capsule color
Muir stain or Welchs

light violet

4

capsule and background color
India ink or nigrosin


capsule: Unstained
bg: black

5

capsule and background color
Congo red

capsule: Unstained
bg: blue

6

capsule and background color
Anthony’s stain

capsule: Unstained
bg: purple

7

granule color
Albert stain





Blue black

8

Neisser stain granule color

Dark blue

9

Lindegren’s stain granule color

Reddish brown

10

Loeffler’s alkaline methylene blue (LAMB) granule color

dark blue

11

Wayson’s stain

bipolar bodies (appear to be spore) are red

12

Stain for flagella (4)

• Leifson stain
• Gray’s method
• Silver technique
• Caesares Gil

13

endospore color
Fulton Schaeffer’s method



green

14

endospore color
Dorner’s stain

red

15

endospore color
Wirtz Conklin

green

16

Stain for Rickettsia (3)

• Castaneda – blue
• Machiavelo – red
• Giemsa – blue

17

Stain for Chlamydia (3)

• Gimenez – inclusion bodies are red
• Machiavello
• Giemsa

18

Stain for spirochetes
Fontana Tribondeau

dark brown or black spirochetes

19

Levaditi Silver Impregnation

– black

20

India Ink negative stain

– unstained spirochetes, black background

21

Stain for mycoplasma
• Dienes stain –


blue

22

Stain for cell wall
• Victoria blue dye –

blue

23

Stain for DNA
• Feulgen stain –

greenish or with green tinge

24

chemical used in Kinyoun's method

tergitol

25

function of tergitol

reduces surface tension, promotes penetration or attachment

26

alters decolorizer by weakening it to differentiate these 2 species: M. tuberculosis and M. smegma

Pappenheim’s method

27

method that differentiates M. tuberculosis and M. leprae

Baumgarten's method

28

stains M. leprae in tissue sections

Wadefite Technique

29

staining technique for bacteria in tissue sections

Brown and Brenn technique

30

modified Grams stain

Brown and Brenn technique

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