Flashcards in MYCOVIRO - CH 60 of book part 4 Deck (33)
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1
broad based yeast
Blastomyces dermatitidis
2
circular to pyriform (dumb-bell) conidia, lollipop conidiophores
Blastomyces dermatitidis
3
“prickly state” – tufts of hyphae projects upward
Blastomyces dermatitidis
4
Exoantigen test or nucleic acid probe testing: presence of A band
Blastomyces dermatitidis
5
direct exam of sputum or lung aspirate
Coccidioides immitis
6
observe non-budding thick-walled spherule
Coccidioides immitis
7
spherule either contains granular material or endospores
Coccidioides immitis
8
alternate arthroconidia – arthroconidia separated by disjunctor cells
Coccidioides immitis
9
small septate racquet hyphae; rectangular to barrel-shaped arthroconidia (stains with
lactophenol cotton or aniline blue)
Coccidioides immitis
10
wet preparation: sterile saline + petrolatum, incubated overnight
Coccidioides immitis
11
empty, collapsed “ghost” spherules may be present (bc of release of endospores)
Coccidioides immitis
12
delicate cobweb-like growth
Coccidioides immitis
13
Some have aerial hyphae, others have vegetative hyphae (adhere to agar surface)
Coccidioides immitis
14
fluffy white, greenish discoloration on blood agar
Coccidioides immitis
15
should be examined only under Level 3 BSC; culture should be sealed with tape to prevent
infection; screw-capped tubes must be used
Coccidioides immitis
16
Histoplasma capsulatum
- Stain and specimen ??
Wright- or Giemsa-stained bone marrow specimens
17
found intracellularly in mononuclear cells as small round yeast cells
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Histoplasma capsulatum
18
small, intertwined hyphae
large, spherical or pyriform, smooth-walled macroconidia (characteristic tuberculate with age)
echinulate macroconidia (?)
Histoplasma capsulatum
19
to remove contamination in H. capsulatum:
uses yeast extract/ phosphate medium and a drop of conc NH4OH
20
recovered from blood culture within 8 days
Histoplasma capsulatum
21
white, fluffy mold that turns brown to buff with age
tufts of hyphae project upward
Histoplasma capsulatum
22
mariner’s wheel, mickey mouse cap
Paracoccidioides brasiliensis
23
heaped with crater formations, wrinkled, moist, yeastlike colonies o covered with short aerial mycelium and turns tan to brown with age
Paracoccidioides brasiliensis
24
test for definitive identification of Paracoccidioides brasiliensis
Exoantigen test:
25
no budding cells
extensively branched and highly septate hyphae
may be detected in peripheral blood smears in disseminated infection o mycelial forms produce brush-like conidiophores (penicillus)
Penicillium marneffei
26
observe small, yeastlike cells with septa; reveals annelid formation
Penicillium marneffei
27
produces blue-green to yellowish colonies on Sabouraud’s o soluble red to maroon pigment diffuses into agar
Penicillium marneffei
28
Stain: exudate aspirated from unopened subcutaneous nodules or from open draining lesions
Sporothrix schenckii
29
small, round to cigar-shaped yeast cells
Sporothrix schenckii
30
