Bacte Lec - Entero Part 2

Question 1

LIA: Lysine decarboxylase +

Answer 1

Purple/purple

Question 2

LIA: Lysine decarboxylase -

Answer 2

Purple/yellow

Question 3

LIA: Deaminase positive

Answer 3

Red/Yellow

Question 4

LIA: Blackening

Answer 4

H2S positive

Question 5

IMViC

Answer 5

Indole Methyl red voges proskauer citrate test

Question 6

tryptone is present in what cm

Answer 6

tryptone broth

Question 7

when tryptone is utilized, what is the by product?

Answer 7

indole

Question 8

+ result in indole tests

Answer 8

red ring when overlayed with Kovac’s or Ehrlich’s reagent

Question 9

what is the Kovac’s or Ehrlich’s reagent

Answer 9

PDAB (Para-diethylaminobenzaldehyde or Para-dimethylaminobenzaldehyde)

Question 10

if tryptone broth is not available, what to use?

Answer 10

SIM

Question 11

enzyme in indole tests

Answer 11

tryptophanase

Question 12

• result in indole tests

Answer 12

yellow or brown ring [no tryptophanase]

Question 13

glucose by products

Answer 13

acetoin

pyruvic acid

Question 14

meaning of K/K on LIA

Answer 14

decarboxylation (purple/purple)

Question 15

meaning of R/A on LIA

Answer 15

deamination (red/yellow)

Question 16

when acetoin is oxidized by air or KOH, it is transformed to

Answer 16

diacetyl

Question 17

diacetyl in MR test is determined by adding

Answer 17

VP reagent

Question 18

what is the VP Reagent

Answer 18

5% alpha naphthol in absolute methyl alcohol

40% KOH w/ 0.3% creatine

Question 19

+ result in VP test

Answer 19

pink-red color [unstable]

Question 20

MR & VP cannot be both [what result + or -]

Answer 20

cannot be both +

Question 21

color of methyl red in acidic solutions

Answer 21

red

Question 22

mixed acid fermentation makes the medium

Answer 22

acidic, pH less than 4.4

Question 23

pH range of methyl re

Answer 23

4-6

Question 24

+ result in MR test

Answer 24

red color

Question 25

culture medium of citrate utilizaton test

Answer 25

simmon citrate agar

Question 26

principle of citrate utilizaton test

Answer 26

based on degradation of citrate

Question 27

indicator of sca

Answer 27

bromthymol blue

Question 28

+ result in citrate utilizaton test

Answer 28

blue [means citrate is degraded]

Question 29

- result in citrate utilizaton test

Answer 29

green

Question 30

rep organism for gram (-)

Answer 30

Escherichia coli

Question 31

rapid urease producing organisms

Answer 31

Proteus, Campylobacter, H. pylori

Question 32

best way to differentiate enterobacter and klebsiella

Answer 32

motility test

Question 33

best motility test

Answer 33

hanging drop

Question 34

identify organism LF GMS on emb IMViC Test: ++--

Answer 34

escherichia

Question 35

identify organism LF dark purple on emb IMViC Test: --++

Answer 35

enterobacter | klebsiella

Question 36

``` less mucoid urease negative motile w/o capsule center [fish eye] ```

Answer 36

enterobacter

Question 37

mucoid more urease + nonmotile w/ capsule

Answer 37

klebsiella

Question 38

nonlactose fermenter, spreading/swarming

Answer 38

Pseudomonas | Proteus/Morganella

Question 39

nonlactose fermenter, spreading/swarming, pigmented,

Answer 39

pseudomonas

Question 40

reaction of pseudomonas in TSI

Answer 40

K/K

Question 41

nonlactose fermenter, spreading/swarming, non-pigmented,

Answer 41

Proteus | Morganella

Question 42

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A

Answer 42

Morganella morganii

Question 43

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +

Answer 43

P. vulgaris or P. mirabilis

Question 44

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +, indol +

Answer 44

P. vulgaris

Question 45

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +, indol -

Answer 45

P. mirabilis

Question 46

odor of proteus

Answer 46

burned gunpowder

Question 47

odor of pseudomonas

Answer 47

fruity

Question 48

+ result in urease test

Answer 48

red color

Question 49

- result in urease test

Answer 49

salmon/yellow color