CM LEC - Synovial fluid Part 2 Flashcards Preview

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Flashcards in CM LEC - Synovial fluid Part 2 Deck (94)
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1

most frequently performed cell
count on synovial fluid

Total leukocyte count

2

When is RBC count requested

when there is traumatic tap

3

what to do if there is a delay in processing/testing of sx

refrigerate

4

Very viscous fluid may be pretreated by adding
(2)

a pinch amount of hyaluronidase to 0.5mL of fluid OR
one drop of 0.05% hyaluronidase in PO4 buffer per mL of fluid
& incubate at 37°C for 5mins

5

dilution of clear fluids

none

6

dilution is needed in what kind of fluids (2)

bloody or turbid fluids

7

diluting fluid not suitable for synovial fluid cell count

traditional WBC dil. fluid or acetic acid

8

Traditional WBC diluting fluid cannot be used – acetic
acid will cause formation of ___

mucin clot

9

recommended diluent for synovial fluid cell counts

normal saline

10

To lyse RBCs use: (2)

hypotonic saline (0.3%) or saline that
contains saponin as diluent

11

stain WBC nuclei permitting separation of RBCs & WBCs on mixed specimens

Methylene blue + normal saline

12

disadv of automated cell counters (2)

Highly viscous fluid may block the apertures
Presence of debris & tissue cells may falsely elevate
counts

13

differential counts must be performed on __
OR
specimen preparation used for diff count

 Cytocentrifuged preparations or thinly smeared slides

14

in diff count, fluid should be incubated with ______ prior to
slide preparation

hyaluronidase

15

 Primary cells seen in normal synovial fluid:

 Monocytes
 Macrophages
 Synovial tissue cells

16

diff count
neutrophil %

17

diff count
lymphocyte %

18

Increased neutrophils means there is

septic condition

19

Increases lymphocytes = means

nonseptic inflammation

20

in diff count, in normal and abnormal specimen, cells may appear

more vacuolated than on a blood smear

21

lipid droplets may be present following ____

crash injuries

22

found in cases of pigmented villonodular synovitis

hemosiderin granules

23

Hemosiderin granules found in cases of ___

pigmented villonodular synovitis

24

other cell abnormalities (4)

- eosinophils
- LE cells
- Reiter cells
- RA cells or ragocytes

25

(vacuolated macrophages with ingested
neutrophils)

Reiter cells

26

(neutrophils with small, dark, cytoplasmic granules that consist of precipitated rheumatoid factor)

RA cells or ragocytes

27

Additional crystals present:
-basic calcium phosphate
-calcified cartilage degeneration

hydroxyapatite

28

Additional crystals present
following injections

corticosteroids

29

Additional crystals present
renal dialysis patients

calcium oxalate crystals

30

Crystal examination should be performed soon after fluid collection to ensure crystals are not affected by changes in (2)

temperature & pH

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