Bacte Lec - Culture Media Lecture Flashcards
1
Q
bacterial nutritional requirements (3)
A
water
energy source
carbon source
2
Q
types of organisms based on their source of energy
A
phototrophs
chemotrophs
3
Q
types of organisms based on their carbon source
A
heterotroph
autotrophs
4
Q
types of chemotroph
A
lithotrophs
organotrophs
5
Q
types of organisms based on their carbon source
require CO2
A
autotrophs
6
Q
types of organisms based on their carbon source
require organic compounds
A
heterotrophs
7
Q
types of organisms based on their energy source
require light
A
phototrophs
8
Q
types of organisms based on their energy source
require chemicals
A
chemotrophs
9
Q
prevention of bacterial growth by water removal
A
lyophilization
10
Q
organisms which need co2
A
capnophiles
11
Q
examples of capnophiles
A
Haemophilus influenzae
Neisseria gonorrhoea
12
Q
energy source: light
C source: CO2
A
photoautotrophs
13
Q
energy source: light
C source: organic compounds
A
photoheterotrophs
14
Q
energy source: inorganic compounds
C source: CO2
A
chemoautotrophs
15
Q
energy source: organic compounds
C source: organic compounds
A
chemoheterotrophs
16
Q
photoautotrophs
energy source
A
light
17
Q
photoheterotrophs
energy source
A
light
18
Q
chemoautotrophs
energy source
A
inorganic compounds
19
Q
chemoheterotrophs
energy source
A
organic compounds
20
Q
chemoheterotrophs
c source
A
organic compounds
21
Q
chemoautotrophs
c source
A
co2
22
Q
photoheterotrophs
c source
A
organic compounds
23
Q
photoautotrophs
c source
A
CO2
24
Q
Representative bacteria of photoautotrophs
A
cyanobacteria
photosynthetic bacteria
algae
25
Representative bacteria of photoheterotrophs
photosynthetic bacteria
26
Representative bacteria of chemoheterotrophs
protozoa, fungi, bacteria
27
Representative bacteria of chemoautotrophs
sulfur, iron, ammonia oxidizing bacteria
| methane producing bacteria
28
essential elements needed by microorganisms [TOM]
Metals
Trace Elements
Organic Growth Factors
29
metals needed by microorganisms
K, Mg, Ca, Fe
30
Trace elements needed by microorganisms
Molybdenum
Copper
Cobalt
Zinc
31
Organic growth factors
purine and pyrimidine
amino acids
vitamins
32
elements of culture media [6]
```
free of inhibitory substances
proper ph
sufficient moisture
desired consistency
initially sterile
right nutrients
```
33
classification of CM according to composition
synthetic, chemically defined
| non-synthetic, chemically undefined, complex
34
classification of CM according to composition
| specific substances can be identified
synthetic, chemically defined
35
examples of synthetic, chemically defined
nutrient agar/broth
36
classification of CM according to composition
| specific substances cannot be identified
non-synthetic, chemically undefined, complex
37
ex of non-synthetic, chemically undefined, complex
yeast extract, beef extract
38
digested proteins rich in protens, amino acids, nitrogen and carbon source
peptone
39
form of dehydrated culture media
granular
| powder
40
glassware for preparation of plated cm
erlenmeyer flask
41
glassware for preparation of tubed cm
beaker
42
classification of CM according to physical state
solid
liquid
semi-solid
43
agar composition in solid cm
1.5 -2 %
44
agar compostion in semi solid
0.5 - 1%
45
complex polysaccharide [marine algae]
agar
46
temp of liq agar
100c
47
temp of solid agar
40C
48
adv of albumin as solidifying agent
coagulates at high temperature
49
solidifying agent: coagulates at high temperature
albumin
50
solidifying agent: diagnostic medium
gelatin
51
3 solidifying agent
agar
albumin
gelatin
52
ex of semisolid medium
SIM
53
semisolid medium which determines motility or ability to produce hydrogen sulfide and also for indole detection
Sulfide Indole Motility
54
agar composition of liquid medium
0%
55
merely a solidifying agent
agar agar
56
if you are to prepare 100 ml of a certain culture media, how much in g of agar agar should you add
1.5 - 2g
57
classif of cm according to function
```
>general isolation/supportive/simple/basal/ordinary
>enriched/nonselective
>selective
>differential
>enrichment
```
58
classif of cm according to function
| non-fastidious bacteria
general isolation/supportive/simple/basal/ordinary
59
classif of cm according to function
| fastidious bacteria
enriched/nonselective
60
nutrient supplements placed in an enriched or nonselective culture medium
blood
ascitic fluid
serum
61
difference between enriched and enrichment
enriched - promotes growth of all organisms
| enrichment - promotes growth of organism of interest
62
difference between CAP and BAP
BAP - blood is added when the basal medium has cooled
| CAP - blood is added when the basal medium is hot, which causes lysis of cells, attributed to the chocolate color
63
temp when to added Blood in nutrient agar
40-45C
64
basal medium of bap and cap
nutrient agar
65
types of blood [3]
5% defibrinated sheep's blood
horse blood
outdated human blood
66
types of blood
| for fastidious like Haemophilus
5% defibrinated sheep's blood
67
types of blood
| for differentiation of various types of hemolysis
5% defibrinated sheep's blood
68
Media with solid and liquid
biphasic
69
type of hemolysis
| no hemolysis
gamma hemolysis
70
type of hemolysis
| complete hemolysis
beta hemolysis
71
type of hemolysis
| partial hemolysis
alpha hemolysis
72
type of hemolysis
| greenish discoloration that surrounds a bacterial colony
alpha hemolysis
73
___ hemolysis is characteristic of Streptococcus pyogenes and some strains ofStaphylococcus aureus.
beta
74
___ hemolysis is characteristic of Streptococcus pneumonia
alpha
75
type of blood
| gives erratic or consistent results
horse blood
76
disadv of outdated human blood
contains: antibacterial substances, excess citrate ions, other substances (antibodies)
77
agar which contains heated blood or enzyme treated blood
chocolate agar
78
CAP contains what type of blood
heated blood or enzyme treated blood
79
anticoagualant for blood
citrate phosphate dextrose [CPD] or CPD A
80
prevents clotting
defibrination
81
what to place in an EF to prevent clotting/defibrination
paper clips or marbles [fibrin will adhere to these]
82
classif of cm according to function
| distinguishes between organisms based on appearance on the medium
differential medium
83
classif of cm according to function
| distinguishes between lactose fermenters and non-lactose fermenters
differential medium
84
classif of cm according to function
| contains indicators
differential medium
85
classif of cm according to function
| enhances bacterial growth of desired bacteria
selective medium
86
inhibitory substances in selective medium
```
bile salts
sodium azide
sodium desoxycholate
potassium tellurite
gentian violet
antibiotics
```
87
blood agar can be enriched and differential
enriched: addition of blood
differential: differentiates hemolytic reactions
88
classif of cm according to function
| tinsdale agar
selective
89
classif of cm according to function
| MSA
selective
90
classif of cm according to function
| SSA
selective
91
classif of cm according to function
| LJ
selective
92
Components of transport medium
buffer and salts
93
special cm
| maintain viability w/o altering concentration
transport medium
94
special cm
| temporary storage of chemical sx
transport medium
95
special culture medium
| enhances growth of bacteria that may be overgrown by competitors
enrichment broth
96
special cm
| layers of living cells
cell line/tissue culture
97
special cm
| series of battery test medium
biochemical test media
98
examples of biochemical test media [3]
enterotube
API Test OR API Medium
Packaged Test System
99
series of color reactions matched with a color chart with an interpretation
biochemical test media
100
added when an organism is suspected to be an anaerobe
mineral oil
101
color of nonfermenter
red
102
color of fermenter
yellow
103
displacement of agar interpretation
gas production capability of organism
104
blackening of butt interpretation
H2S production
105
small tubes used to detect gas production
Durham tube
106
growth on the bottom of thioglycollate broth
anaerobe
107
growth on the surface of thioglycollate broth
aerobe
108
growth on the middleof thioglycollate broth
microaerophilic
109
indicates that organism is toxic
cytopathic effect (CPE) or cell death
