Flashcards in CC Lab - Spectrophotometry Deck (28):
1
major categories of analytical techniques
spectrometry
luminescence
electroanalytical method
chromatography
2
main principle of spectro
beer's law/beer-lambert's law
3
3 major methods for calculation of unknown solution
direct comparison/ratio of standard to unknown
use of standard curve
use of molar absorptivity value
4
source of reagent for exp
reagent kit
5
3 substances found in reagent
catalyst
enzyme
buffer
6
type of spectrophotometer in the lab
Humalyzer Primus
7
old spectrophotometer
ALS 2000
8
Conventional parts of spectrophotometer (6)
exciter lamp
variable slit diaphragm
monochromator
sample holder
photodetector
read out device
9
parts of spectrophotometer
light source
exciter lamp
10
parts of spectrophotometer
separates radiant energy
monochromator
11
parts of spectrophotometer
isolate narrow band of light
variable slit diaphragm
12
wavelength of glucose
500 nm
13
units of wavelength
nm or mu or 10^-9m
14
parts of spectrophotometer
holds smaple
sample holder/cuvetter
15
shape of cuvette
round or square
16
parts of spectrophotometer
reads amount of transmitted light then converts it to an electrical signal
photodetector
17
parts of spectrophotometer
galvanometer
converts electric signal to a readable data
read out device
18
keypad (4 commands)
ESC
Enter
Rinse
Paper
19
rinsing solution
triple distilled water
20
wait how many min after turning on the spectrophotometer
5 min
21
2 types of operating procedure
Kinetic or rate method
Endpoint method
21
2 types of operating procedure
Kinetic or rate method
22
Operating procedure for ALS 2000 Spectrophotometer
Endpoint method
23
No sx
To set reading at 0
Blank
24
Blank solution with reagent
Reagent blank
25
Blank soln with only the sample, no rgt
Serum or sample blank
26
Empty test tube to set reading at zero
Air blank
27