CC Lab - Spectrophotometry

Question 1

major categories of analytical techniques

Answer 1

spectrometry
luminescence
electroanalytical method
chromatography

Question 2

main principle of spectro

Answer 2

beer’s law/beer-lambert’s law

Question 3

3 major methods for calculation of unknown solution

Answer 3

direct comparison/ratio of standard to unknown
use of standard curve
use of molar absorptivity value

Question 4

source of reagent for exp

Answer 4

reagent kit

Question 5

3 substances found in reagent

Answer 5

catalyst
enzyme
buffer

Question 6

type of spectrophotometer in the lab

Answer 6

Humalyzer Primus

Question 7

old spectrophotometer

Answer 7

ALS 2000

Question 8

Conventional parts of spectrophotometer (6)

Answer 8

exciter lamp
variable slit diaphragm
monochromator
sample holder
photodetector
read out device

Question 9

parts of spectrophotometer

light source

Answer 9

exciter lamp

Question 10

parts of spectrophotometer

separates radiant energy

Answer 10

monochromator

Question 11

parts of spectrophotometer

isolate narrow band of light

Answer 11

variable slit diaphragm

Question 12

wavelength of glucose

Answer 12

500 nm

Question 13

units of wavelength

Answer 13

nm or mu or 10^-9m

Question 14

parts of spectrophotometer

holds smaple

Answer 14

sample holder/cuvetter

Question 15

shape of cuvette

Answer 15

round or square

Question 16

parts of spectrophotometer

reads amount of transmitted light then converts it to an electrical signal

Answer 16

photodetector

Question 17

parts of spectrophotometer
galvanometer
converts electric signal to a readable data

Answer 17

read out device

Question 18

keypad (4 commands)

Answer 18

ESC
Enter
Rinse
Paper

Question 19

rinsing solution

Answer 19

triple distilled water

Question 20

wait how many min after turning on the spectrophotometer

Answer 20

5 min

Question 21

2 types of operating procedure

Answer 21

Kinetic or rate method

Endpoint method

Question 21

2 types of operating procedure

Answer 21

Kinetic or rate method

Question 22

Operating procedure for ALS 2000 Spectrophotometer

Answer 22

Endpoint method

Question 23

No sx

To set reading at 0

Answer 23

Blank

Question 24

Blank solution with reagent

Answer 24

Reagent blank

Question 25

Blank soln with only the sample, no rgt

Answer 25

Serum or sample blank

Question 26

Empty test tube to set reading at zero

Answer 26

Air blank

Question 27

Tool for precision which BEHAVES LIKE A SPECIMEN

Answer 27

Controls