GPHT LAB - Infiltration Flashcards Preview

Botany > GPHT LAB - Infiltration > Flashcards

Flashcards in GPHT LAB - Infiltration Deck (80)
Loading flashcards...
0

filling up of empty spaces cavities using an impregnating medium

infiltration

1

why impregnate

to give tx a firm consistency

2

best and most common infiltrating medium

paraffin wax

3

MP of pure PW

56C

4

__ not be overheated

PW

5

PW requires __ hrs of infiltration

.

6

PW must be __ prior to use

filtered

7

coarse filter for PW

Green's # 904

8

PW requires what equipment

paraffin oven

9

if PW is reused, heat at __ to remove water

100C

10

PW can be used how many times

2x

11

disadv of PW

not for fatty tissues

12

PW Substitute
paraffin + polymer

paraplast

13

MP of paraplast

56-57C

14

Quite similar to paraplast but MP is 56-58C

Embeddol

15

with a rubber what PW Substitute

tissue mat

16

harder than ordinary PW but MP = 46-48C

Esterwax

17

PW Substitute
Soluble in 95% EtOH

Esterwax

18

MP of Esterwax

46-48C

19

Ex. of water-soluble waxes

carbowax

20

Adv of carbowax

no need for dehydration and clearing

21

disadv of carbowax

expensive, tissue sections dissolved during flotation

22

microtome for carbowax

base sledge or sliding

23

Methods of Paraffin impregnation
Changes of PW, transfer tissue from one container to another

manual processing

24

Methods of Paraffin impregnation
performs steps 2-5

automatic tissue processing

25

Methods of Paraffin impregnation
fastest, performed at negative atmospheric pressure

vacuum method

26

celloidin also called

colloidion

27

wax for hollow large tx tend to collapse

celloidin

28

no heating required

celloidin

29

time for processing when using celloidin

days to weeks

Decks in Botany Class (324):