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GPHT LAB - Infiltration Flashcards

0
Q

why impregnate

A

to give tx a firm consistency

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1
Q

filling up of empty spaces cavities using an impregnating medium

A

infiltration

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2
Q

best and most common infiltrating medium

A

paraffin wax

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3
Q

MP of pure PW

A

56C

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4
Q

__ not be overheated

A

PW

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5
Q

PW requires __ hrs of infiltration

A

.

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6
Q

PW must be __ prior to use

A

filtered

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7
Q

coarse filter for PW

A

Green’s # 904

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8
Q

PW requires what equipment

A

paraffin oven

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9
Q

if PW is reused, heat at __ to remove water

A

100C

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10
Q

PW can be used how many times

A

2x

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11
Q

disadv of PW

A

not for fatty tissues

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12
Q

PW Substitute

paraffin + polymer

A

paraplast

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13
Q

MP of paraplast

A

56-57C

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14
Q

Quite similar to paraplast but MP is 56-58C

A

Embeddol

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15
Q

with a rubber what PW Substitute

A

tissue mat

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16
Q

harder than ordinary PW but MP = 46-48C

A

Esterwax

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17
Q

PW Substitute

Soluble in 95% EtOH

A

Esterwax

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18
Q

MP of Esterwax

A

46-48C

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19
Q

Ex. of water-soluble waxes

A

carbowax

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20
Q

Adv of carbowax

A

no need for dehydration and clearing

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21
Q

disadv of carbowax

A

expensive, tissue sections dissolved during flotation

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22
Q

microtome for carbowax

A

base sledge or sliding

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23
Q

Methods of Paraffin impregnation

Changes of PW, transfer tissue from one container to another

A

manual processing

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24
Methods of Paraffin impregnation | performs steps 2-5
automatic tissue processing
25
Methods of Paraffin impregnation | fastest, performed at negative atmospheric pressure
vacuum method
26
celloidin also called
colloidion
27
wax for hollow large tx tend to collapse
celloidin
28
no heating required
celloidin
29
time for processing when using celloidin
days to weeks
30
2 methods for celloidin | for brain and bone tx
wet celloidin method
31
2 methods for celloidin | eyes
drycelloidin method
32
for enzyme studies, heat sensitive substances
gelatin
33
no dehydration and clearing required (2)
gelatin | carbowax
34
preservative of gelatin (to prevent mold growth)
1% phenol
35
hard medium: can cut very thick sections
plastic/resins
36
for preparing ultra-thin and EM sections
plastic/resins
37
widest application, for light and EM
Epoxy resins
38
why impregnate
to give tx a firm consistency
39
best and most common infiltrating medium
paraffin wax
40
MP of pure PW
56C
41
__ not be overheated
PW
42
PW requires __ hrs of infiltration
Less than 24 hours
43
PW must be __ prior to use
filtered
44
coarse filter for PW
Green's # 904
45
PW requires what equipment
paraffin oven
46
if PW is reused, heat at __ to remove water
100C
47
PW can be used how many times
2x
48
disadv of PW
not for fatty tissues
49
PW Substitute | paraffin + polymer
paraplast
50
MP of paraplast
56-57C
51
Quite similar to paraplast but MP is 56-58C
Embeddol
52
with a rubber what PW Substitute
tissue mat
53
harder than ordinary PW but MP = 46-48C
Esterwax
54
PW Substitute | Soluble in 95% EtOH
Esterwax
55
MP of Esterwax
46-48C
56
Ex. of water-soluble waxes
carbowax
57
Adv of carbowax
no need for dehydration and clearing
58
disadv of carbowax
expensive, tissue sections dissolved during flotation
59
microtome for carbowax
base sledge or sliding
60
Methods of Paraffin impregnation | Changes of PW, transfer tissue from one container to another
manual processing
61
Methods of Paraffin impregnation | performs steps 2-5
automatic tissue processing
62
Methods of Paraffin impregnation | fastest, performed at negative atmospheric pressure
vacuum method
63
celloidin also called
colloidion
64
wax for hollow large tx tend to collapse
celloidin
65
no heating required
celloidin
66
time for processing when using celloidin
days to weeks
67
2 methods for celloidin | for brain and bone tx
wet celloidin method
68
2 methods for celloidin | eyes
drycelloidin method
69
for enzyme studies, heat sensitive substances
gelatin
70
no dehydration and clearing required (2)
gelatin | carbowax
71
preservative of gelatin (to prevent mold growth)
1% phenol
72
hard medium: can cut very thick sections
plastic/resins
73
for preparing ultra-thin and EM sections
plastic/resins
74
widest application, for light and EM
Epoxy resins
75
Rarely used infiltrating medium
Gelatin
76
Why use preservative for gelatin
To prevent growth of molds
77
Does not require dehydration and clearing (2)
Carbowax | Gelatin
78
Components of plastic/resins
Acrylic Polyester Glycerol Epoxy resins
79
Component of plastic/resin infiltrating medium with widest application, for LM and EM
Epoxy resin

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