Bacte Lec - Quiz 2 Flashcards
- media supplemented with serum or sugar to prevent osmotic pressure
- capable of reverting to normal form (Residual) peptidoglycan
L-forms
– physical mordant in AF stain
Steam or heat
chemical mordant present in carbolfuchsin
Phenol –
2 Methods of Acid- Fast Staining
- Ziehl-Neelsen method– hot, steaming for 5 min
2. Kinyoun method – cold, more than 60 min
may be a substitute for methylene blue as counterstain in AF Staining
Malachite green
– placed on top of the smear before flooding with carbolfuchsin
Filter paper
function of filter paper
-prevents rapid evaporation of dye (dye is not allowed to dry)
cells commonly found in clinical specimens because it is the first to react during a bacterial infection
Neutrophils –
– round, spherical, shaped like berries or grapes
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coccus
identify species
(flattened on one side like a coffee bean)
Neisseria gonorrhoea
multiple planes and form grapelike clusters or broad sheets
staphylococci
2 planes remain in groups of 4 (ex. Micrococcus tetragena)
• tetrads:
3 planes and remain attached in cubelike groups of 8
• sarcinae
example of species with sarcinae arrangement
Sarcina lutea
remain in pairs after dividing
diplococci:
example of species with diplococci arrangement
(Streptococcus pneumonia/Diplococcus pneumonia)
type of cocci arrangement
divide and remain in chain like pattern
streptococci:
-usually round but can be oval, elongated, flattened on one side
cocci
example of tetrad
Micrococcus tetragena
example of streptococci
streptococcus pyogenes
Arrangements of bacilli
singly, chains, pairs
Gram-positive long bacilli, a common cause of bacterial contamination in laboratories
Bacillus subtilis
arrangement of bacillus
may align themselves side by side
palisading
example of palisading
Corynebacteria or Corynebacterium diphtheria
