Hema Lab - Cell Counting Flashcards Preview

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Flashcards in Hema Lab - Cell Counting Deck (67)
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1

2 Methods of Cell Counting

manual method
automation

2

2 basis/method of manual method

turbidimetric technique
hemacytometer

3

type of manualmethod that estimates by means of turbidimetry

turbidimetric technique

4

components of hemacytometer

counting chamber
thoma pipette (RBC, WBC)

5

Types of Counting Chamber

Fuchs-Rosenthal
Improved Neubauer
Neubauer Counting Chamber
Speirs-Levy

6

pipets used for blood dilution

thoma pipettes

7

parts of a thoma pipette

stem
bead
bulb

8

distinct calibrations/lines on thoma pipette

0.5
1

9

highest mark on RBC Thoma pipette

101

10

highest mark on WBC Thoma pipette

11

11

bore size of RBC pipette

smaller

12

bore size of WBC pipette

larger

13

number of drops to discard on RBC pipette

5-6

14

number of drops to discard on WBC pipette

2-3

15

volume capacity of RBC pipette

100 units

16

volume capacity of WBC pipette

10 units

17

Why do we need to discard first few drops from the Thoma pipette

The blood is in the bulb, while the diluting fluid is in the stem.

18

purpose of bead

aids in mixing

19

standard dilution for RBC count

1:200

20

standard dilution for RBC count

1:20

21

type of CC
larger than Neubauer CC

Fuchs- Rosenthal

22

type of CC
used in low cell count:eosinohil, spinal fluid, leukopenic blood count, basophil count

Fuchs-Rosenthal

23

Fuchs-Rosenthal uses (4)

low cell count:
eosinohil,
spinal fluid,
leukopenic blood count,
basophil count

24

measurement of primary square of F-R CC

4x4 mm

25

depth of Fuchs-Rosenthal

0.2 mm

26

How many ruled areas in F-R

2 (upper and lower)

27

Primary square of F-R has how many secondary squares

16

28

Measurement of secondary squares of F-R

1x1 mm or 1 sq mm

29

Secondary square of F-R has how many tertiarysquares

1/16 sq mm (1/4 x 1/4)

30

Number of tertiary squares per ruled area in F-R

256

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