Hema Lab - Cell Counting Flashcards by Carmina Mislang

2 Methods of Cell Counting

manual method

automation

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2 basis/method of manual method

turbidimetric technique

hemacytometer

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type of manualmethod that estimates by means of turbidimetry

turbidimetric technique

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components of hemacytometer

counting chamber
thoma pipette (RBC, WBC)

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Types of Counting Chamber

Fuchs-Rosenthal
Improved Neubauer
Neubauer Counting Chamber
Speirs-Levy

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pipets used for blood dilution

thoma pipettes

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parts of a thoma pipette

stem
bead
bulb

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distinct calibrations/lines on thoma pipette

0.5

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highest mark on RBC Thoma pipette

101

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highest mark on WBC Thoma pipette

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bore size of RBC pipette

smaller

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bore size of WBC pipette

larger

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number of drops to discard on RBC pipette

5-6

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number of drops to discard on WBC pipette

2-3

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volume capacity of RBC pipette

100 units

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volume capacity of WBC pipette

10 units

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Why do we need to discard first few drops from the Thoma pipette

The blood is in the bulb, while the diluting fluid is in the stem.

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purpose of bead

aids in mixing

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standard dilution for RBC count

1:200

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standard dilution for RBC count

1:20

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type of CC

larger than Neubauer CC

Fuchs- Rosenthal

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type of CC

used in low cell count:eosinohil, spinal fluid, leukopenic blood count, basophil count

Fuchs-Rosenthal

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Fuchs-Rosenthal uses (4)

low cell count:
eosinohil, 
spinal fluid, 
leukopenic blood count, 
basophil count

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measurement of primary square of F-R CC

4x4 mm

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depth of Fuchs-Rosenthal

0.2 mm

How many ruled areas in F-R

2 (upper and lower)

Primary square of F-R has how many secondary squares

Measurement of secondary squares of F-R

1x1 mm or 1 sq mm

Secondary square of F-R has how many tertiarysquares

1/16 sq mm (1/4 x 1/4)

Number of tertiary squares per ruled area in F-R

256

Number of tertiary squares in one F-R CC

512

No. of ruled areas in S-L CC

4 ruled areas

number of SECONDARY squares in each ruled area in S-L CC

10 squares

measurement of secondary squares in S-L CC

1x1 mm or 1 sq mm

depth of S-L CC

0.2 MM

T or F: All CC have a secondary square that measures 1x2 mm

True

measurement of primary square in S-L CC

5mm x 2 mm or 10 sq mm

number of primary squares in S-L CC

4 primary squares

number of tertiary squares in each secondary square in S-L CC

measurement of tertiary square in S-L CC

1/16 sq mm or 0.25 x 0.25 mm

number of tertiary squares in one ruled area of S-L CC

160

number of tertiary squares in one S-L CC

640

number of secondary squares in one S-L CC

CC used for all manual cellular counts using blood & other body fluids

Improved Neubauer Counting Chamber

measurement of primary square in INCC

9 sq mm or 3x3 mm

number of secondary squares in INCC

measurement of secondary square in INCC

1x1 mm or 1 sq mm

number of tertiary squares in central secondary square of INCC

Measurement of one tertiary square in the central secondary square INCC

1/25 sq mm or 1/5 x 1/5

number of smallest squares in one tertiary square in INCC

square used in platelet count using INCC

entire central secondary square

uses of central secondary square of INCC

platelet and RBC count

square/s used in RBC count using INCC

4 corner tertiary squares in the central secondary square + the central tertiary square

number of tertiary squares in 4 corner secondary square of INCC

measurement of one tertiary square in 4 corner secondary square of INCC

1/4 mm x 1/4 mm or 1/16 sq mm

total number of smallest squares in central secondary square of INCC

400

total number of smallest squares in central secondary square used in RBC count using INCC

similar to INCC except that the central secondary square is divided into 16 tertiary squares only

NCC | Neubauer Counting Chamber

total number of smallest squares in NCC

16 X 16 = 256 smallest square

total number of smallest squares in central secondary square used in RBC count USING NCC

area counted in INCC in Standard RBC count

number of tertiary squares used in RBC count x area of each tertiary square 5 x 1/25 = 1/5 sq mm

area counted in NCC in Standard RBC count

number of tertiary squares used in RBC count x area of each tertiary square 5 x 1/16 = 5/16 sq mm

formula in volume calculations

area x depth

depth of INCC

0.1 mm

area from 0.1 to 101

mixing chamber or bulb of RBC pipette

area from 0.0 to 1.0

stem both RBC and WBC pipette

area from 1.0 to 11

mixing chamber or bulb of WBC pipette