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Botany > BACTE LEC - Entero Part 3 > Flashcards

BACTE LEC - Entero Part 3 Flashcards

1
Q

principle of phenylalanine test

A

Deamination of phenylalanine to phenylpyruvic acid

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2
Q

positive rxn of the phenylalanine test

A

Positive (green color in the syneresis fluid

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3
Q

ONPG meaning

A

O-nitrophenyl-beta-D- galactopyranoside

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4
Q

test for Slow or late lactose fermenter

A

O-nitrophenyl-beta-D-galactopyranoside

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5
Q

only one that is ONPG positive

Salmonella serotype

A

Salmonella arizonae

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6
Q

rxn of citrobacter in ONPG

A

+

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7
Q

E. coli is a normal flora of the

A

GI tract

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8
Q

Closely related to Shigella (DNA homology)

A

Escherichia coli

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9
Q

“Montezuma’s revenge” “turista” Traveller’s diarrhea

A

Enterotoxigenic E. coli (ETEC)

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10
Q

diseases caused by ETEC

A

“Montezuma’s revenge”
“turista”
Traveller’s diarrhea

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11
Q

E. coli strain

Severe epidemic diarrhea = cholera Fever, headache, vomiting, chills

A

Enterotoxigenic E. coli (ETEC)

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12
Q

E. coli strain

Disease = Shigellosis

A

Enteroinvasive E. coli (EIEC)

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13
Q

Invasion of intestinal epithelium

E. coli strain

A

Enteroinvasive E. coli (EIEC)

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14
Q

E. coli strain

Fever, cramps, bloody stool w/ RBCs, neutrophil and mucus

A

Enteroinvasive E. coli (EIEC)

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15
Q

Verotoxin causes what diarrhea

A

bloody diarrhea

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16
Q

Sorbitol negative SMAC

A

Enterohemorrhagic E. coli (EHEC)

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17
Q

Verotoxic E. coli (VTEC)
0157 H7



A

Enterohemorrhagic E. coli (EHEC)

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18
Q

E. coli strain

Hemolytic uremic syndrome

A

Enterohemorrhagic E. coli (EHEC)

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19
Q

E. coli strain

Hemorrhagic colitis

A

Enterohemorrhagic E. coli (EHEC)

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20
Q

strain number of Enterohemorrhagic E. coli (EHEC)

A

0157 H7

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21
Q

Resembles E. coli
Lactose negative & nonmotile
Shigellosis (bacillary dysentery)

A

Shigella

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22
Q

Shigella: lactose (-) or (+)?

A

Lactose negative

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23
Q

Motility of Shigella

A

nonmotile

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24
Q

disease caused by shigella

A

shigellosis

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25
bacillary dysentery
shigellosis
26
with fishy odor
shigella
27
enteric bacteria which causes Bloody, mucoid, diarrheic stool w/ pus cells & fishy odor
shigella
28
significant shigella species [4]
S. sonnei S. dysenteriae S. flexneri S. boydii
29
Shigella spp Most prevalent in the US Neurotoxin & enterotoxin Non-mannitol fermenter
S. dysenteriae
30
``` Shigella spp. Beta galactosidase (ONPG +) ODC + ```
S. sonnei
31
Motile & ODC Positive Large amount of gas from glucose 
Enterobacter
32
Enterobacter spp | Predominant isolate UTI & wound infection
E. cloacae
33
Enterobacter spp Resembles E. cloacae Yellow pigmented Neonatal sepsis
E. sakazakii
34
``` Short & nonmotile encapsulated Large, mucoid colonies Strings out when touched by the needle ```
Klebsiella
35
Klebsiella | Bacterial pneumonias
K. Pneumoniae
36
Kleb spp. | Blood & stool culture Indole positive
K. oxytoca
37
Kleb spp. Atrophy of the nasal mucous membranes w/ fetid odor Blood & stool
K. ozanae
38
Kleb spp. Rhinoscleroma Destructive granuloma of the nose & pharynx 
K. rhinoscleromatis
39
impt spp of klebsiella [poor]
pneumonia oxytoca ozanae rhinoscleromatis
40
impt spp of serratia [morl]
marcescens liquefaciens odorifera rubidaea
41
Opportunistic pathogens Deoxyribonuclease, lipase, gelatinase red pigment
serratia
42
serratia spp. | Pneumonia & septicemia
S. marcescens
43
serratia spp. | Rancid, potato-like odor
S. odorifera
44
serratia spp. | Ferment arabinose
S. liquefaciens
45
Negative to Lactose, sucrose, sorbitol Citrate, DNAse, lipase
Hafnia alvei
46
Contaminated IV fluids Yellow pigment Negative to ADH, LDH, ODC
Pantoea agglomerans
47
number of serotypes of salmonella
2200
48
very complex genus
salmonella
49
classification of salmonella serotypes
Kauffman - White
50
enteric [typhoid] fever lasts for __
1 week
51
appearance of salmonella on BSA and BGA
Metallic colonies with black ring on bsa
52
sx for disease by salmonella - 1st and 2nd week
blood
53
sx for disease by salmonella - 3rd and 4th week
urine
54
sx for disease by salmonella - indefinite
stool
55
clinical diseases induced by salmonella
septicemia enteric fever gastroenteritis
56
antigen test for salmonella
widal test
57
o antigen in widal test meaning
active infection
58
h antigen in widal test meaning
past immunization or infection
59
Salmonella spp w/c causes Enteric fever Typhoid & paratyphoid
S. typhi
60
Salmonella spp w/c causes | septicemia
S. choleraesius
61
Salmonella spp w/c causes | gastroenteritis
S. typhimurium | S. enteritidis
62
salmonella test Amebocytes of horse crab (visible gel) endotoxin
linulus test
63
Ballooning of the intestinal tract or ileal loop (accumulation of fluid) enterotoxin
ileal loop test
64
2 genera that citrobacter resembles
salmonella | e. coli
65
2 impt spp of citrobacter
freundii | diversus
66
tribe of Proteus Providencia Morganella
proteeae
67
tribe proteeae Rapid urease activity “swarming” motility on BA 
proteus
68
Source of GI infection W ound infections abscesses Trauma or accidents (aquatic environment) Biochemically similar to E. coli
Edwardsiella tarda
69
Abundant H2S in TSI agar Lactose negative LDC & ODC positive ADH negative
Edwardsiella tarda
70
size/appearance of yersinia on MAC
pinpoint
71
morphology of yersinia
coccobacilli
72
yersinia spp motile at RT
y. pestis
73
genus: nonmotile at 37C
Yersinia
74
yersinia spp which causes enterocolitis Ingestion of contaminated water, pork, beef or milk products Fecal-oral transmission
Y. enterocolitis
75
Ferments mannitol and appears as red, “bull’s eye” colonies w/ colorless halo CIN medium
yersinia enterolitica
76
types of plague by y. pestis
bubonic, septicemic, pneumonic
77
causes plague [black death]
y. pestis
78
vector of y. pestis
flea [through a bite]
79
yersinia with safety pin appearance
yersinia pestis
80
yersinia | “Stalactite patterns”
yersinia pestis
81
yersinia spp. | ferments both sucrose & sorbitol
yersinia enterolitica
82
yersinia spp. | fails to ferment both sucrose & sorbitol
yersinia pseudotuberculosis
83
Yersinia spp. Wild animals & game fowl Lymphadenitis in children ODC negative Fails to ferment both sucrose & sorbito
Y. pseudotuberculosis
83
Citrobacter species H2S + KCN +
Freundii
84
Citrobacter species | Indol +
C diversus

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