Flashcards in GPHT LAB - Apparatus I Deck (48):
1
machine on which thin sections are cut
microtome
2
process of using a microtome
microtomy
3
principle of microtomy
pawl is brought into contact with a ratchet wheel, which is connected to a mill head micrometer screw. this action turns the ratchet wheel, which in turn rotates the screw. By this means the block is moved towards the knife at a predetermined thickness
4
3 essential parts of a microtome
1) knife and knife carrier
2) block holder or chuck
3) ratchet feed wheel, adjustment screws, pawl
5
An essential part of the microtome used for actual cutting of tissue sections
knife and knife carrier
6
An essential part of the microtome where the tissue is held in position
block holder or chuck
7
An essential part of the microtome used to line up the tissue block in proper position with the knife, adjust the proper thickness of the tissue for successive sections
ratchet feed wheel, adjustment screws, pawl
8
An essential part of the microtome adjusted or manipulated during microtomy
ratchet feed wheel, adjustment screws, pawl
9
types of microtome (5)
rotary (minot)
rocking
freezing
ultra-thin
sliding or base sledge
10
microtome invented by Caldwell Trefall
Rocking
11
microtome invented by Queckett
Freezing
12
microtome invented by Minot
Rotary (Minot)
13
microtome invented in 1881
Rocking
14
microtome invented in 1885 - 1886
Rotary (Minot)
15
microtome invented in 1848
freezing
16
microtome developed by Adams
Sliding or Base Sledge
17
microtome developed in 1789
Sliding or Base Sledge
18
microtome also known as Cambridge
Rocking
19
Rocking Microtome also known as
Cambridge
20
simplest of all microtomes
Rocking
21
what type of microtome in which the tissue block being mounted on the end of a spring poised rocking arm, the knife held in horizontal position, blade face up and slightly inclined towards the block
Rocking
22
disadvantage of rocking microtome
will not produce GOOD serial sections
23
ribbons of how many sections are produced w/ a rocking microtome
60-90
24
thickness of the sections produced by a rocking micrometer in um
10-12 um
25
what type of microtome
relatively small length of knife is available for use; dangerously placed w/ the blade up
rotary (minot)
26
what type of microtome
not suitable for cutting large blocks
rotary (minot)
27
what type of microtome
for cutting serial sections
rotary (minot)
28
what type of microtome
most commonly used; present in the lab
rotary (minot)
29
what type of microtome
able to section paraffin-embedded sections
rotary (minot)
30
what type of microtome
consists of a central screw by which the block holder is perforated, attached to it is the food pipe carrying carbon dioxide from a cylinder
freezing
31
in the freezing microtome, what allows the release of rapid intermittent burst of carbon dioxide
simple lever-operated valve
32
in the freezing microtome, what is the propellant or freezing agent
carbon dioxide
33
what type of microtome
used for rush frozen sections
freezing
34
what type of microtome
capable of cutting sections at 0.5 micron required for EM
ultra-thin
35
what type of microtome
knife contains selected fragments of broken plate glass
ultra-thin
36
what knife can be used for ultra-thin microtome
special diamond knife
37
what type of microtome
for cutting extremely hard blocks and for frozen sections
sliding or base sledge
38
what type of microtome
first microtome invented
sliding or base sledge
39
microtome knife is made of what?
stainless steel
40
histology technician's too of his profession
microtome knife
41
personal possession of histo tech
microtome knife
42
every technician should be given at least __ good knives
2
43
3 basic shapes of microtome knife
biconcave
plane concave
plane wedge
44
length of biconcave
120 mm
45
microtome knife used for paraffin sections with rocking or rotary type microtome
biconcave
46
microtome knife that can be used on a carbon dioxide freezing microtome
biconcave
47
microtome knife very useful for cutting paraffin tissue blocks
biconcave
48