GPHT LAB - Apparatus I Flashcards Preview

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Flashcards in GPHT LAB - Apparatus I Deck (48)
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1

machine on which thin sections are cut

microtome

2

process of using a microtome

microtomy

3

principle of microtomy

pawl is brought into contact with a ratchet wheel, which is connected to a mill head micrometer screw. this action turns the ratchet wheel, which in turn rotates the screw. By this means the block is moved towards the knife at a predetermined thickness

4

3 essential parts of a microtome

1) knife and knife carrier
2) block holder or chuck
3) ratchet feed wheel, adjustment screws, pawl

5

An essential part of the microtome used for actual cutting of tissue sections

knife and knife carrier

6

An essential part of the microtome where the tissue is held in position

block holder or chuck

7

An essential part of the microtome used to line up the tissue block in proper position with the knife, adjust the proper thickness of the tissue for successive sections

ratchet feed wheel, adjustment screws, pawl

8

An essential part of the microtome adjusted or manipulated during microtomy

ratchet feed wheel, adjustment screws, pawl

9

types of microtome (5)

rotary (minot)
rocking
freezing
ultra-thin
sliding or base sledge

10

microtome invented by Caldwell Trefall

Rocking

11

microtome invented by Queckett

Freezing

12

microtome invented by Minot

Rotary (Minot)

13

microtome invented in 1881

Rocking

14

microtome invented in 1885 - 1886

Rotary (Minot)

15

microtome invented in 1848

freezing

16

microtome developed by Adams

Sliding or Base Sledge

17

microtome developed in 1789

Sliding or Base Sledge

18

microtome also known as Cambridge

Rocking

19

Rocking Microtome also known as

Cambridge

20

simplest of all microtomes

Rocking

21

what type of microtome in which the tissue block being mounted on the end of a spring poised rocking arm, the knife held in horizontal position, blade face up and slightly inclined towards the block

Rocking

22

disadvantage of rocking microtome

will not produce GOOD serial sections

23

ribbons of how many sections are produced w/ a rocking microtome

60-90

24

thickness of the sections produced by a rocking micrometer in um

10-12 um

25

what type of microtome
relatively small length of knife is available for use; dangerously placed w/ the blade up

rotary (minot)

26

what type of microtome
not suitable for cutting large blocks

rotary (minot)

27

what type of microtome
for cutting serial sections

rotary (minot)

28

what type of microtome
most commonly used; present in the lab

rotary (minot)

29

what type of microtome
able to section paraffin-embedded sections

rotary (minot)

30

what type of microtome
consists of a central screw by which the block holder is perforated, attached to it is the food pipe carrying carbon dioxide from a cylinder

freezing

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