2.16 Micro

Question 0

Mechanism of fluroquinolone antibiotics

Answer 0

Causes ds breaks

Question 1

What antibiotics target DNA gyrate

Answer 1

Fluroquinolone (ex: ciprofloxacin)

Question 2

Pyrimidine synthesis pathway

Answer 2

Carbamyl phosphate + aspartate

Carbamyl aspartate

UMP

UTP

CTP

Question 3

Example of an anabolic gene

Answer 3

Histidine

Question 4

Example of a catabolic gene

Answer 4

Lactose

Question 5

Is lac operon regulation pos or neg

Answer 5

Neg

Question 6

Concept of polarity

Answer 6

Nonsense mutation in lacZ will prevent lacY expression as well because lacZ is upstream from lacY

Question 7

Concept of catabolism repression

Answer 7

Increase in glucose will decrease cAMP which decreases ability of CAP-cAMP to help lac operon expression

Question 8

What are the 2 components of the 2 component regulatory systems

Answer 8

Sensor or histidine kinase

Response regulator

Question 9

Resistance to what antibiotic uses the 2 component system

Answer 9

Vancomycin (resistance to vancomycin when it’s present because it activates the 2 component system and allows bac to express vancomycin resistant genes. Also needs to replace pentapeptide with lactose)

Question 10

A nucleic acid that catalyzes reactions is called _____ and give an example

Answer 10

Ribozyme

Ribosome

Question 11

Growth rate of bac depends on

Answer 11

Number of ribosomes