Wys 1 Flashcards
(22 cards)
How do aldehyde fixatives work
Crosslink (add covalent bonds between proteins)
Process that prevents autolysis
Fixation
How do alcohol fixatives work
Precipitate / denature (reduce solubility of protein by disrupting hydrophobic interactions between tertiary structures)
How does neutral buffered formalin work
Cross links proteins and amino acids (lysine)
What does formalin not preserve
mRNA, miRNA, DNA
pH needed for fixation
4-9
Size of specimen for fixation
1-4 mm
Volume of fixative
15-20 times more than tissue volume
Duration of fixation
1 hour per mm
Tissue removed after fixation
Ions
Glycogen
Small proteins
Nucleus acids
What chemical causes lipids and mucous to be leeched out?
Paraffin
Hematoxylin
Color
Charge
Phile
Attaches to..
Blue
+
Base
DNA
Eosin
Color
Charge
Phile
Attaches to..
Acid
Protein/Cytoplasm/Fibers
Which due requires a mordant, and what is a mordant, what is used
Hematoxylin
Intermediate link between tissue and dye
Al
What microscope uses a dicrotic mirror
Fluorescent
What light microscope generates a 3D image?
Confocal scanning
What are the benefits of a 2 photon microscope
Can go deeper in tissue
Doesn’t kill tissue
What optical process allows you to see malaria secretions glow blue?
Third harmonic generation
How is SEM different than TEM?
Looks at surface
Uses backscattered electrons
What are the two portions called in freeze fracture?
Exoplasmic leaflet
Protoplasmic leaflet
What electron microscope(s) generate 3D images?
Serial block face scanning EM
Scanning EM
Which microscopy studies vibrations and rigidity of rumors?
Atomic force microscopy
